{"id":4114,"date":"2019-09-23T18:34:14","date_gmt":"2019-09-23T09:34:14","guid":{"rendered":"http:\/\/163.180.4.222\/lab\/?p=4114"},"modified":"2019-09-23T18:34:14","modified_gmt":"2019-09-23T09:34:14","slug":"glowing-dna-label-illuminates-a-cells-fine-details","status":"publish","type":"post","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=4114","title":{"rendered":"Glowing DNA label illuminates a cell\u2019s fine details"},"content":{"rendered":"

 <\/p>\n

 <\/p>\n

Fluorescent tag can be affixed to proteins or genetic structures of interest.<\/h5>\n

 <\/p>\n

 <\/p>\n

\"Nerve<\/p>\n

DNA tags could help scientists to study ion channels (yellow), which allow specific ions to pass through the membrane of nerve cells (green). Credit: Patrick Landmann\/SPL<\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

\n

A glowing tag made of DNA can be used to label a single target molecule in a cell.<\/p>\n

Today\u2019s advanced microscopes allow physicists to image individual molecules, and even arrange them in simple patterns. But such equipment is not very useful for biologists, because biological structures are complex and easily damaged by microscope probes.<\/p>\n

Mingjie Dai and Peng Yin at Harvard Medical School in Boston, Massachusetts, and their colleagues developed a technique that uses fluorescent DNA strands to label biological molecules in a cell. First the label\u2019s DNA binds to a matching strand on a target molecule. Then a fluorescent-dye particle attached to the label glows under illumination. This \u2018blink\u2019 switches on a laser, which triggers a chemical element embedded in the DNA label to form a tight bond with the target molecule.<\/p>\n

The researchers tagged nanometre-scale synthetic DNA structures with a 65% success rate. They also used the technique to tag protein filaments called microtubules inside cultured cells.<\/p>\n

The tags could be used to ferry activating molecules to cell-membrane proteins called ion channels, which are crucial for transmitting nerve-cell messages. This would reveal the workings of an individual channel in a cell, the authors say.<\/p>\n

 <\/p>\n

 <\/p>\n<\/div>\n

\n

Nature Chem.<\/i>\u00a0(2019)<\/a><\/p>\n<\/div>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

(\uc6d0\ubb38: \uc5ec\uae30<\/a>\ub97c \ud074\ub9ad\ud558\uc138\uc694~)<\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

    Fluorescent tag can be affixed to proteins or genetic structures of interest.     DNA tags could help scientists to study ion channels(more…)<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[33,34,29,30],"tags":[],"class_list":["post-4114","post","type-post","status-publish","format-standard","hentry","category-do-biology","category-lets-do-chemistry","category-lets-do-science","category-recent-science-news"],"aioseo_notices":[],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack-related-posts":[{"id":1550,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=1550","url_meta":{"origin":4114,"position":0},"title":"DNA tags used to image sugar-bearing proteins on cells","author":"biochemistry","date":"September 4, 2018","format":false,"excerpt":"\u00a0 \u00a0 (\uc6d0\ubb38) \u00a0 \u00a0 Methods for imaging sugars attached to proteins \u2014 the protein glycoforms \u2014 are of interest because glycoforms affect protein movement and localization in cells. A versatile approach is now reported that uses DNA as molecular identity tags. \u00a0 \u00a0 The attachment of sugar molecules to\u2026","rel":"","context":"In "Let's Do Biology!"","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":2250,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2250","url_meta":{"origin":4114,"position":1},"title":"Artificial cells gain communication skills","author":"biochemistry","date":"December 3, 2018","format":false,"excerpt":"\u00a0 \u00a0 No biologist would mistake the microscopic \u201ccells\u201d that chemical biologist Neal Devaraj and colleagues are whipping up at the University of California, San Diego (UCSD), for the real thing. Instead of the lipid membrane that swaddles our cells, these cell mimics wear a coat of plastic\u2014polymerized acrylate. And\u2026","rel":"","context":"In "Let's Do Biology!"","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":4090,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=4090","url_meta":{"origin":4114,"position":2},"title":"Emerging uses of DNA mechanical devices","author":"biochemistry","date":"September 18, 2019","format":false,"excerpt":"\u00a0 \u00a0 Modern machines, which are composed of force-generating motors, force sensors, and load-bearing structures, enabled the industrial revolution and are foundational to human civilization. Miniature micromachines are used in countless devices including cell phone microphones, implantable biosensors, and car and airplane accelerometers. Further miniaturization to the nanometer scale would\u2026","rel":"","context":"In "Let's Do Chemistry!"","block_context":{"text":"Let's Do Chemistry!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=34"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":3448,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=3448","url_meta":{"origin":4114,"position":3},"title":"Pinpointing a spatial address for RNA profiles in tissues","author":"biochemistry","date":"May 4, 2019","format":false,"excerpt":"\u00a0 \u00a0 Knowing the gene-expression pattern of individual cells can unlock their identity. A refined method for generating cellular RNA profiles offers a way to obtain such data at a high level of spatial resolution in intact tissues. \u00a0 \u00a0 Monitoring messenger RNA in cells is a way to gather\u2026","rel":"","context":"In "Essays on Science"","block_context":{"text":"Essays on Science","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=32"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":2670,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2670","url_meta":{"origin":4114,"position":4},"title":"Revealing a microbial carcinogen","author":"biochemistry","date":"February 15, 2019","format":false,"excerpt":"\u00a0 \u00a0 The microbiota in the human gastrointestinal system is predicted to produce hundreds of unique small molecules and secondary metabolites that may influence host health and disease (1). Many such molecules are produced by sophisticated multienzymatic assembly lines that are encoded by bacterial biosynthetic gene clusters. One class of\u2026","rel":"","context":"In "Let's Do Biology!"","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":2582,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2582","url_meta":{"origin":4114,"position":5},"title":"Technologies to watch in 2019","author":"biochemistry","date":"January 29, 2019","format":false,"excerpt":"\u00a0 \u00a0 From higher-resolution imaging to genome-sized DNA molecules built from scratch, the year ahead looks exciting for life-science technology. \u00a0 An automated bioreactor system for growing yeast, which can be used to investigate synthetic genomes \u2014 one area poised to make big strides this year.Credit: Tim Llewellyn\/Ginkgo Bioworks \u00a0\u2026","rel":"","context":"In "Essays on Science"","block_context":{"text":"Essays on Science","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=32"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]}],"jetpack_sharing_enabled":false,"jetpack_shortlink":"https:\/\/wp.me\/p9Xo1j-14m","_links":{"self":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts\/4114","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4114"}],"version-history":[{"count":1,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts\/4114\/revisions"}],"predecessor-version":[{"id":4115,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts\/4114\/revisions\/4115"}],"wp:attachment":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4114"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=4114"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=4114"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}