{"id":3996,"date":"2019-08-05T21:45:44","date_gmt":"2019-08-05T12:45:44","guid":{"rendered":"http:\/\/163.180.4.222\/lab\/?p=3996"},"modified":"2019-08-05T21:45:44","modified_gmt":"2019-08-05T12:45:44","slug":"a-dynamic-view-of-chemotherapy-effectiveness","status":"publish","type":"post","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=3996","title":{"rendered":"A dynamic view of chemotherapy effectiveness"},"content":{"rendered":"<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h5>Chemotherapy can halt cancer by causing cells to enter a non-dividing state called senescence, but sometimes it causes tumour cells to proliferate. It now seems that the dynamics of the protein p21 governs which of these fates occurs.<\/h5>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>Chemotherapy usually works by inducing DNA damage that leads to cell death. However, rather than dying after chemotherapy, some tumour cells enter an inactive state, termed senescence, in which they are alive but have permanently stopped dividing<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-02336-7?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR1\" data-track=\"click\" data-action=\"anchor-link\" data-track-label=\"go to reference\" data-track-category=\"references\">1<\/a><\/sup>. Although senescence in normal cells drives ageing and tissue degeneration<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-02336-7?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR2\" data-track=\"click\" data-action=\"anchor-link\" data-track-label=\"go to reference\" data-track-category=\"references\">2<\/a><\/sup>, cancer-therapy-induced senescence is associated with positive clinical outcomes<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-02336-7?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR3\" data-track=\"click\" data-action=\"anchor-link\" data-track-label=\"go to reference\" data-track-category=\"references\">3<\/a><\/sup>. Understanding the factors that drive the senescence of tumour cells might thus aid the development of new anticancer treatments.\u00a0<a href=\"https:\/\/doi.org\/10.1016\/j.cell.2019.05.041\" data-track=\"click\" data-label=\"https:\/\/doi.org\/10.1016\/j.cell.2019.05.041\" data-track-category=\"body text link\">Writing in\u00a0<i>Cell<\/i><\/a>, Hsu\u00a0<i>et al<\/i>.<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-02336-7?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR4\" data-track=\"click\" data-action=\"anchor-link\" data-track-label=\"go to reference\" data-track-category=\"references\">4<\/a><\/sup>\u00a0shed light on a previously unknown aspect of how chemotherapy-induced entry into senescence is controlled.<\/p>\n<p>Although much progress has been made in uncovering factors that drive senescence, the processes that ultimately commit cells to this fate are poorly understood. A growing body of evidence indicates that the regulation of commitment to enter senescence is complex. The mere presence of factors associated with triggering this cell fate is not in itself sufficient to provide an \ufffd\ufffd\ufffdon switch\ufffd\ufffd\ufffd for senescence.<\/p>\n<p>&nbsp;<\/p>\n<p><a href=\"https:\/\/www.nature.com\/articles\/d41586-017-08652-0\" data-track=\"click\" data-track-label=\"recommended article\"><img decoding=\"async\" class=\"recommended__image\" src=\"https:\/\/media.nature.com\/w400\/magazine-assets\/d41586-019-02336-7\/d41586-019-02336-7_17033822.jpg\" \/><\/a><\/p>\n<aside class=\"recommended pull pull--left sans-serif\" data-label=\"Related\">\n<p class=\"recommended__title serif\">Escape from senescence boosts tumour growth<\/p>\n<\/aside>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>The protein p21 is probably best known for its role in blocking cell division by inhibiting protein complexes called cyclin-dependent kinases. If DNA damage occurs, p21 activity halts cell division and growth<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-02336-7?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR5\" data-track=\"click\" data-action=\"anchor-link\" data-track-label=\"go to reference\" data-track-category=\"references\">5<\/a><\/sup>, giving cells time for DNA repair and thereby preventing such damage from having catastrophic cellular consequences. There is evidence that p21 can induce senescence during chemotherapy<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-02336-7?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR6\" data-track=\"click\" data-action=\"anchor-link\" data-track-label=\"go to reference\" data-track-category=\"references\">6<\/a><\/sup>. Yet, paradoxically, some research suggests that the protein can promote cancer-cell division after chemotherapy<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-02336-7?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR7\" data-track=\"click\" data-action=\"anchor-link\" data-track-label=\"go to reference\" data-track-category=\"references\">7<\/a><\/sup>. One possible explanation for this discrepancy is that the abundance and dynamics of p21 after chemotherapy have a key role in determining whether cancer cells enter senescence or divide.<\/p>\n<p>To test this idea, Hsu and colleagues developed a microscopy system to study thousands of individual, cultured human lung and colon cancer cells that had been treated with a DNA-damaging chemotherapy drug. The authors monitored the abundance of p21 by tagging it with a fluorescent protein, and also tracked the progression of the various stages of the cell cycle. In contrast to previous research suggesting that high levels of p21 invariably lead to either cell growth or senescence<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-02336-7?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR5\" data-track=\"click\" data-action=\"anchor-link\" data-track-label=\"go to reference\" data-track-category=\"references\">5<\/a><\/sup><sup>,<\/sup><sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-02336-7?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR7\" data-track=\"click\" data-action=\"anchor-link\" data-track-label=\"go to reference\" data-track-category=\"references\">7<\/a><\/sup>, the authors describe a complex, but unifying, picture of how p21 levels relate to cell fate. Hsu\u00a0<i>et al<\/i>. noted that if chemotherapy resulted in an initial rise in p21 levels followed by a decline to low levels, cell division, rather than senescence, occurred (Fig. 1). Cancer cells that entered senescence after drug treatment initially had a low level of p21 that gradually rose to a high level.<\/p>\n<p>&nbsp;<\/p>\n<figure class=\"figure\">\n<div class=\"embed intensity--high\">\n<div class=\"embed intensity--high\"><img decoding=\"async\" class=\"figure__image\" src=\"https:\/\/media.nature.com\/w800\/magazine-assets\/d41586-019-02336-7\/d41586-019-02336-7_17034280.jpg\" alt=\"\" data-src=\"\/\/media.nature.com\/w800\/magazine-assets\/d41586-019-02336-7\/d41586-019-02336-7_17034280.jpg\" \/><\/div>\n<\/div><figcaption>\n<p class=\"figure__caption sans-serif\"><span class=\"mr10\"><b>Figure 1 | Levels of p21 protein and cancer-cell fate after chemotherapy.<\/b>\u00a0<b>a<\/b>, Hsu\u00a0<i>et al<\/i>.<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-02336-7?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR4\" data-track=\"click\" data-action=\"anchor-link\" data-track-label=\"go to reference\" data-track-category=\"references\">4<\/a><\/sup>\u00a0found that, when human cancer cells grown\u00a0<i>in vitro<\/i>\u00a0were treated with chemotherapy drugs at a stage in the cell cycle before DNA replication, two types of cell fate were observed. In some cells, p21 levels rose and the cells entered a permanent state of non-division termed senescence. In other cells, after an initial rise in the level of p21, the protein returned to a low level and the cells divided. The authors describe this p21-dependent switch in cell fate as being affected by a \ufffd\ufffd\ufffdGoldilocks zone\ufffd\ufffd\ufffd (yellow), in which the levels and dynamics of the protein after drug treatment must be \ufffd\ufffd\ufffdjust right\ufffd\ufffd\ufffd for cells to be able to halt the cell cycle, repair DNA and then continue to divide.\u00a0<b>b<\/b>, By contrast, if cells received drug treatment during or after DNA replication, p21 levels gradually rose and cells entered senescence. (Graphs based on Fig. 3 of ref. 4, showing just the 72-hour window during and after drug treatment.)<\/span><\/p>\n<\/figcaption><\/figure>\n<p>Hsu and colleagues suggest that there is a \ufffd\ufffd\ufffdGoldilocks zone\ufffd\ufffd\ufffd for proliferation \ufffd\ufffd\ufffd a level of p21 that is \ufffd\ufffd\ufffdjust right\ufffd\ufffd\ufffd to allow tumour cells to divide after chemotherapy. How might p21 dynamics control cell fate in this way? Chemotherapy drugs are most damaging to DNA if given to cells at the cell-cycle stage at which DNA replication occurs<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-02336-7?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR8\" data-track=\"click\" data-action=\"anchor-link\" data-track-label=\"go to reference\" data-track-category=\"references\">8<\/a><\/sup>. It might therefore be expected that cells given chemotherapy during DNA replication would have higher levels of p21 than would cells treated before DNA replication occurs. Yet, surprisingly, Hsu and colleagues found that cells treated during DNA replication had high levels of DNA damage but low levels of p21, and that levels of p21 then increased over time. By contrast, drug treatment before DNA replication resulted in a rapid rise in p21 expression that, depending on the individual cell, either returned to a low level or rose further.<\/p>\n<p>How do some cancer cells that have undergone drug-induced DNA damage revert to having low levels of p21 expression and gain the ability to divide? The authors propose a model that incorporates dynamic regulation of p21 expression and the level of DNA damage. They suggest that cell fate after chemotherapy shows a property termed bistability \ufffd\ufffd\ufffd cells are poised to follow one of two fates.<\/p>\n<p>In this scenario, at the cell-cycle stage before DNA replication, if cells express intermediate levels of p21 and small fluctuations occur in signals identifying DNA damage due to chemotherapy, such fluctuations might promote either the rapid induction or decline of p21 expression, driving cells to, respectively, enter senescence or divide.<\/p>\n<p>&nbsp;<\/p>\n<aside class=\"recommended pull pull--left sans-serif\" data-label=\"Related\"><a href=\"https:\/\/www.nature.com\/articles\/nature22493\" data-track=\"click\" data-track-label=\"recommended article\"><img decoding=\"async\" class=\"recommended__image\" src=\"https:\/\/media.nature.com\/w400\/magazine-assets\/d41586-019-02336-7\/d41586-019-02336-7_15297290.jpg\" \/><\/a><\/p>\n<p class=\"recommended__title serif\">Tools to eliminate senescent cells<\/p>\n<\/aside>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>However, when cells undergo DNA replication, a stage of the cell cycle at which DNA-damage signals are higher than normal (because errors can occur during DNA replication), only a slight increase in the level of p21 would be enough to establish a stable state of high p21 that would lead to senescence. Thus, the Goldilocks zone is defined by the level of p21 and DNA damage, and determines whether cells divide after chemotherapy. To assess the clinical relevance of this finding, the dose and drug dependency of p21 dynamics during chemotherapy should be examined in detail.<\/p>\n<p>These results raise the possibility that targeting cancer cells at specific cell-cycle stages would produce major differences in the cellular response to chemotherapy, with cells targeted during or shortly after DNA replication being more likely to enter senescence than cells targeted before replication. This model should be investigated further. Taking such an approach in the clinic would pose challenges, however, given that tumours contain a mixture of cell populations that are at different cell-cycle stages. One strategy to tackle this could be to direct cells towards the specific cell-cycle stage at which chemotherapy would be most effective. The authors found that if cells were treated with a small molecule that triggers DNA replication, senescence occurred more commonly than cell division after chemotherapy.<\/p>\n<p>Another challenge will be to identify the optimal outcome for a given cancer following chemotherapy. Although senescence might be an ideal response for certain tumours, others that are more prone to dying in response to cellular damage might be more effectively treated by inducing cell death rather than by triggering senescence.<\/p>\n<p>Hsu and colleagues\ufffd\ufffd\ufffd work provides a detailed foundation for understanding what governs the fate of cancer cells after chemotherapy. Now it is time to build on this progress, to determine whether strategies can be found that maximize the effectiveness of our current arsenal of anticancer agents.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>(\uc6d0\ubb38: <a href=\"https:\/\/www.nature.com\/articles\/d41586-019-02336-7?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29\">\uc5ec\uae30<\/a>\ub97c \ud074\ub9ad\ud558\uc138\uc694~)<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>&nbsp; &nbsp; Chemotherapy can halt cancer by causing cells to enter a non-dividing state called senescence, but sometimes it causes tumour cells to proliferate. It<a href=\"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=3996\" class=\"more-link\">(more&#8230;)<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[33,34,29,30],"tags":[],"class_list":["post-3996","post","type-post","status-publish","format-standard","hentry","category-do-biology","category-lets-do-chemistry","category-lets-do-science","category-recent-science-news"],"aioseo_notices":[],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack-related-posts":[{"id":4699,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=4699","url_meta":{"origin":3996,"position":0},"title":"Senescent cells feed on their neighbours","author":"biochemistry","date":"October 31, 2019","format":false,"excerpt":"\u00a0 Chemotherapy-treated cancer cells that enter a non-dividing state called senescence can nevertheless boost cancer growth. The finding that these cells eat neighbouring cells reveals a mechanism that enables senescent cells to persist. \u00a0 \u00a0 Multicellular life requires individual cells to cooperate in a way that benefits the organism. Cells\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":2451,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2451","url_meta":{"origin":3996,"position":1},"title":"Chemotherapy and tumor immunity","author":"biochemistry","date":"January 7, 2019","format":false,"excerpt":"\u00a0 \u00a0 A large increase in the incidence of cancers has been predicted for the coming years, with the number of cases worldwide rising from 15 million to 24 million between 2015 and 2035 (1). The current revolution in cancer treatment\u2014cancer immunotherapy\u2014is based on the mobilization of the immune system\u2026","rel":"","context":"In &quot;Essays on Science&quot;","block_context":{"text":"Essays on Science","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=32"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":2625,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2625","url_meta":{"origin":3996,"position":2},"title":"Inhibition of \u2018jumping genes\u2019 promotes healthy ageing","author":"biochemistry","date":"February 8, 2019","format":false,"excerpt":"\u00a0 \u00a0 DNA sequences called retrotransposons can copy themselves and reintegrate at new sites in the genome, causing damage. It now seems that inhibiting this process can prevent age-related health decline in mice. \u00a0 Old1\u00a0and diseased2\u00a0tissues often contain cells that have entered a state called senescence, in which they stop\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":4790,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=4790","url_meta":{"origin":3996,"position":3},"title":"Small molecule combats cancer-causing KRAS protein at last","author":"biochemistry","date":"November 11, 2019","format":false,"excerpt":"\u00a0 \u00a0 A molecule has now been characterized that acts to inhibit a cancer-causing form of KRAS protein and stimulate the immune system. The inhibitor is one of the first of its kind to show anticancer activity in the clinic. \u00a0 \u00a0 Mutations in the gene\u00a0KRAS\u00a0are the most frequent drivers\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":3933,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=3933","url_meta":{"origin":3996,"position":4},"title":"Flower power as human cancer cells compete with normal cells","author":"biochemistry","date":"July 27, 2019","format":false,"excerpt":"\u00a0 \u00a0 Cells compete for survival during development. It emerges that mammalian cells on a path to form a tumour express specific versions of the protein Flower when they vie for survival with surrounding normal cells. \u00a0 \u00a0 In multicellular organisms, cells usually communicate with each other in a peaceful\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":1366,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=1366","url_meta":{"origin":3996,"position":5},"title":"Mini-tumours turn immune cells into cancer fighters","author":"biochemistry","date":"August 14, 2018","format":false,"excerpt":"\u00a0 \u00a0 (\uc6d0\ubb38) \u00a0 \u00a0 Tumour \u2018organoids\u2019 in lab dishes (left) were seeded with tissue removed from a human lung tumour (right). Credit: K. K. Dijkstra\u00a0et al.\/Cell \u00a0\u00a0 Mini-tumours turn immune cells into cancer fighters Personalized white blood cells attack tumours after incubation with cancer tissue. \u00a0 \u00a0 Miniature tumours\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]}],"jetpack_sharing_enabled":false,"jetpack_shortlink":"https:\/\/wp.me\/p9Xo1j-12s","_links":{"self":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts\/3996","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3996"}],"version-history":[{"count":1,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts\/3996\/revisions"}],"predecessor-version":[{"id":3997,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts\/3996\/revisions\/3997"}],"wp:attachment":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3996"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3996"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3996"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}