{"id":3984,"date":"2019-08-03T23:11:30","date_gmt":"2019-08-03T14:11:30","guid":{"rendered":"http:\/\/163.180.4.222\/lab\/?p=3984"},"modified":"2019-08-03T23:11:30","modified_gmt":"2019-08-03T14:11:30","slug":"enriching-stem-cells-for-gene-editing","status":"publish","type":"post","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=3984","title":{"rendered":"Enriching stem cells for gene editing"},"content":{"rendered":"<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>Gene editing using CRISPR-Cas9 may allow targeted treatment for a variety of genetic diseases. These include inherited abnormalities of \u03b2 hemoglobin, which can be indirectly targeted by increasing the amount of healthy fetal hemoglobin without fully correcting the disease-causing mutation. Humbert\u00a0<em>et al.<\/em>\u00a0used CRISPR-based gene editing to modify hematopoietic stem cells from nonhuman primates, introducing a naturally occurring mutation that increases the amount of fetal hemoglobin. The authors successfully applied this method to a highly enriched population of stem cells in the primate model, suggesting the potential for translating this efficient editing technique to human patients.<\/p>\n<p>&nbsp;<\/p>\n<p id=\"p-2\"><em>Sci. Transl. Med.<\/em>\u00a0<strong>11<\/strong>, eaaw3768 (2019).<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>(\uc6d0\ubb38: <a href=\"https:\/\/science.sciencemag.org\/content\/365\/6452\/458.19?rss=1\">\uc5ec\uae30<\/a>\ub97c \ud074\ub9ad\ud558\uc138\uc694~)<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>&nbsp; &nbsp; Gene editing using CRISPR-Cas9 may allow targeted treatment for a variety of genetic diseases. These include inherited abnormalities of \u03b2 hemoglobin, which can<a href=\"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=3984\" class=\"more-link\">(more&#8230;)<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[33,34,29,30],"tags":[],"class_list":["post-3984","post","type-post","status-publish","format-standard","hentry","category-do-biology","category-lets-do-chemistry","category-lets-do-science","category-recent-science-news"],"aioseo_notices":[],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack-related-posts":[{"id":1313,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=1313","url_meta":{"origin":3984,"position":0},"title":"Did CRISPR really fix a genetic mutation in these human embryos?","author":"biochemistry","date":"August 9, 2018","format":false,"excerpt":"\u00a0 \u00a0 (\uc6d0\ubb38) \u00a0 \u00a0 Researchers provide more evidence for their landmark claim that gene editing rid embryos of a disease mutation \u2014 but scientists are still arguing over the results. \u00a0 \u00a0 Eight-cell embryos injected with the gene editor CRISPR\u2013Cas9.Credit: H. Ma et al.\/Nature \u00a0 \u00a0 \u00a0 Biologists who\u2026","rel":"","context":"In &quot;Essays on Science&quot;","block_context":{"text":"Essays on Science","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=32"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":3524,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=3524","url_meta":{"origin":3984,"position":1},"title":"Stem-cell and genetic therapies make a healthy marriage","author":"biochemistry","date":"May 16, 2019","format":false,"excerpt":"\u00a0 \u00a0 This scientific partnership could fight everything from blood diseases to HIV. \u00a0 A culture from human tissue showing neurons derived from stem cells.Credit: Daniel Schroen\/Cell Applications Inc\/SPL \u00a0 \u00a0 Aside from a 20-second exposure to the outside world at birth, David Vetter spent his entire life cocooned in\u2026","rel":"","context":"In &quot;Essays on Science&quot;","block_context":{"text":"Essays on Science","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=32"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":2952,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2952","url_meta":{"origin":3984,"position":2},"title":"The CRISPR \ufefffix that could combat inherited blood disorders","author":"biochemistry","date":"March 27, 2019","format":false,"excerpt":"\u00a0 Researchers have finally identified a reliable way to edit the genes of blood stem cells. \u00a0 The elongated red blood cells of people with sickle-cell disease can block small blood vessels, reducing the flow of oxygen to nearby tissues. Credit: Eye of Science\/SPL \u00a0 \u00a0 \u00a0 An enhanced version\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":4084,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=4084","url_meta":{"origin":3984,"position":3},"title":"Scientists use gene-edited stem cells to treat HIV \u2014 with mixed success","author":"biochemistry","date":"September 17, 2019","format":false,"excerpt":"\u00a0 \u00a0 Modified cells survived 19 months after transplant into an HIV-positive man in China, but the dose was not enough to reduce his viral load. \u00a0 \u00a0 HIV destroys the body's defences by attacking immune cells.\u00a0Credit: Steve Gschmeissner\/Science Photo Library \u00a0 \u00a0 For the first time, researchers have used\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":3477,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=3477","url_meta":{"origin":3984,"position":4},"title":"Gene editing fixes cystic fibrosis gene in mice before birth","author":"biochemistry","date":"May 9, 2019","format":false,"excerpt":"\u00a0 Administration of nanoparticles\u00a0in utero\u00a0repaired the mutated\u00a0Cftr\u00a0gene \u00a0 The airway of the lungs in cystic fibrosis (pictured here) is clogged with mucus, causing the severe breathlessness seen in this inheritable condition. CNRI\/Science Source \u00a0 \u00a0 Cystic fibrosis, a life-threatening inherited disorder that affects the lungs and other organs, can have\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":2059,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2059","url_meta":{"origin":3984,"position":5},"title":"Towards therapeutic base editing","author":"biochemistry","date":"October 12, 2018","format":false,"excerpt":"\u00a0 \u00a0 \uc6d0\ubb38: \uc5ec\uae30\ub97c \ud074\ub9ad\ud558\uc138\uc694~ \u00a0 Base editors function in mouse fetuses and in the livers of adult mice to treat a genetic disorder. \u00a0 \u00a0 The vast majority of genetic diseases are caused by single-nucleotide mutations rather than chromosomal rearrangements or small insertions or deletions (indels) and hence could\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]}],"jetpack_sharing_enabled":false,"jetpack_shortlink":"https:\/\/wp.me\/p9Xo1j-12g","_links":{"self":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts\/3984","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3984"}],"version-history":[{"count":1,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts\/3984\/revisions"}],"predecessor-version":[{"id":3985,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts\/3984\/revisions\/3985"}],"wp:attachment":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3984"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3984"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3984"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}