{"id":3497,"date":"2019-05-10T11:44:42","date_gmt":"2019-05-10T02:44:42","guid":{"rendered":"http:\/\/163.180.4.222\/lab\/?p=3497"},"modified":"2019-05-10T11:44:42","modified_gmt":"2019-05-10T02:44:42","slug":"how-many-metals-to-oxidize-methane","status":"publish","type":"post","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=3497","title":{"rendered":"How many metals to oxidize methane?"},"content":{"rendered":"

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Methane is an important fuel, but there are few direct transformations to partially oxidized products. Bacteria use metalloenzymes to catalyze methane oxidation to methanol, a reaction of industrial interest. Understanding the metal sites that enable this reaction may inspire new biomimetic catalysts. Ross\u00a0et al.<\/em>\u00a0used spectroscopic measurements to assign two monocopper sites in the enzyme particulate methane monooxygenase. These results differ in part from previous proposals for the location and nuclearity of the metal sites and will prompt rethinking about how this metalloenzyme catalyzes methane oxidation.<\/p>\n

Science<\/em>, this issue p.\u00a0566<\/a><\/p>\n

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(\uc6d0\ubb38: \uc5ec\uae30<\/a>\ub97c \ud074\ub9ad\ud558\uc138\uc694~)<\/p>\n

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Particulate methane monooxygenase contains only mononuclear copper centers<\/h4>\n
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Abstract<\/strong><\/p>\n

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Bacteria that oxidize methane to methanol are central to mitigating emissions of methane, a potent greenhouse gas. The nature of the copper active site in the primary metabolic enzyme of these bacteria, particulate methane monooxygenase (pMMO), has been controversial owing to seemingly contradictory biochemical, spectroscopic, and crystallographic results. We present biochemical and electron paramagnetic resonance spectroscopic characterization most consistent with two monocopper sites within pMMO: one in the soluble PmoB subunit at the previously assigned active site (CuB<\/sub>) and one ~2 nanometers away in the membrane-bound PmoC subunit (CuC<\/sub>). On the basis of these results, we propose that a monocopper site is able to catalyze methane oxidation in pMMO.<\/p>\n

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(\uc6d0\ubb38: \uc5ec\uae30<\/a>\ub97c \ud074\ub9ad\ud558\uc138\uc694~)<\/p>\n

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    Methane is an important fuel, but there are few direct transformations to partially oxidized products. Bacteria use metalloenzymes to catalyze methane oxidation to(more…)<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[33,34,29,30],"tags":[],"class_list":["post-3497","post","type-post","status-publish","format-standard","hentry","category-do-biology","category-lets-do-chemistry","category-lets-do-science","category-recent-science-news"],"aioseo_notices":[],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack-related-posts":[{"id":816,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=816","url_meta":{"origin":3497,"position":0},"title":"Background levels of methane in Mars\u2019 atmosphere show strong seasonal variations","author":"biochemistry","date":"June 10, 2018","format":false,"excerpt":"\u00a0 \u00a0 (\uc6d0\ubb38: \uc5ec\uae30\ub97c \ud074\ub9ad\ud558\uc138\uc694~) \u00a0 Measuring martian organics and methane The Curiosity rover has been sampling on Mars for the past 5 years (see the Perspective by ten Kate). Eigenbrode\u00a0et al.\u00a0used two instruments in the SAM (Sample Analysis at Mars) suite to catch traces of complex organics preserved in\u2026","rel":"","context":"In "Let's Do Chemistry!"","block_context":{"text":"Let's Do Chemistry!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=34"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":3501,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=3501","url_meta":{"origin":3497,"position":1},"title":"Remote control with engineered enzymes","author":"biochemistry","date":"May 10, 2019","format":false,"excerpt":"\u00a0 \u00a0 Many syntheses of organic molecules require that certain carbon-hydrogen bonds are targeted for reaction over others with similar reactivity (1\u20136). This high selectivity to one specific C\u2013H bond is frequently achieved by a remote activating group in the molecule (known as remote functionalization). A particularly attractive group of\u2026","rel":"","context":"In "Let's Do Biology!"","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":3881,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=3881","url_meta":{"origin":3497,"position":2},"title":"Caging cells in functional shells","author":"biochemistry","date":"July 16, 2019","format":false,"excerpt":"\u00a0 \u00a0 Unprotected cells are sensitive to changes in their environment, including temperature, pH, and osmotic pressure. Zhu\u00a0et al.\u00a0have developed a facile route to encapsulating mammalian cells inside functional nanoparticles. 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Plant cells\u2026","rel":"","context":"In "Let's Do Biology!"","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":1220,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=1220","url_meta":{"origin":3497,"position":4},"title":"Scissoring genes with light","author":"biochemistry","date":"July 23, 2018","format":false,"excerpt":"\u00a0 \u00a0 (\uc6d0\ubb38) \u00a0 \u00a0 Nature Chemistry\u00a0volume\u00a010,\u00a0pages\u00a0800\u2013801\u00a0(2018) \u00a0 Enzymes can perform various biological functions because of their delicately and precisely organized structures. Now, simple inorganic nanoparticles with a rationally designed recognition capability can mimic restriction enzymes and selectively cut specific DNA sequences. \u00a0 \u00a0 Designing nanomaterials with properties that enable\u2026","rel":"","context":"In "Let's Do Chemistry!"","block_context":{"text":"Let's Do Chemistry!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=34"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":4927,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=4927","url_meta":{"origin":3497,"position":5},"title":"Peptidic catalysts for macrocycle synthesis","author":"biochemistry","date":"January 7, 2020","format":false,"excerpt":"\u00a0 \u00a0 Many structurally simplified catalysts have been synthesized that mimic the reactivity and efficiency of enzymes. In this context, the numerous transformations catalyzed by the amino acid proline as a catalytic-site mimic helped drive the field of organocatalysis (1). 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