{"id":2795,"date":"2019-03-07T17:26:54","date_gmt":"2019-03-07T08:26:54","guid":{"rendered":"http:\/\/163.180.4.222\/lab\/?p=2795"},"modified":"2019-03-07T17:26:54","modified_gmt":"2019-03-07T08:26:54","slug":"dna-based-communication-in-populations-of-synthetic-protocells","status":"publish","type":"post","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2795","title":{"rendered":"DNA-based communication in populations of synthetic protocells"},"content":{"rendered":"<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h5 id=\"Abs1\" class=\"js-section-title section-title strong position-relative tighten-line-height background-gray-light pt20 pb6 pl0 pr20 standard-space-below small-space-above mq640-pt10 mq640-pb10 mq640-pl20 mq640-mt0 mq640-ml-20 mq640-mr-20 extend-left\">Abstract<\/h5>\n<div id=\"Abs1-content\" class=\"pl20 mq875-pl0 js-collapsible-section\">\n<p>&nbsp;<\/p>\n<p>Developing molecular communication platforms based on orthogonal communication channels is a crucial step towards engineering artificial multicellular systems. Here, we present a general and scalable platform entitled \u2018biomolecular implementation of protocellular communication\u2019 (BIO-PC) to engineer distributed multichannel molecular communication between populations of non-lipid semipermeable microcapsules. Our method leverages the modularity and scalability of enzyme-free DNA strand-displacement circuits to develop protocellular consortia that can sense, process and respond to DNA-based messages. We engineer a rich variety of biochemical communication devices capable of cascaded amplification, bidirectional communication and distributed computational operations. Encapsulating DNA strand-displacement circuits further allows their use in concentrated serum where non-compartmentalized DNA circuits cannot operate. BIO-PC enables reliable execution of distributed DNA-based molecular programs in biologically relevant environments and opens new directions in DNA computing and minimal cell technology.<\/p>\n<\/div>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>(\uc6d0\ubb38: <a href=\"https:\/\/www.nature.com\/articles\/s41565-019-0399-9?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nnano%2Frss%2Fcurrent+%28Nature+Nanotechnology+-+Issue%29\">\uc5ec\uae30<\/a>\ub97c \ud074\ub9ad\ud558\uc138\uc694~)<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>&nbsp; &nbsp; Abstract &nbsp; Developing molecular communication platforms based on orthogonal communication channels is a crucial step towards engineering artificial multicellular systems. Here, we present<a href=\"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2795\" class=\"more-link\">(more&#8230;)<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[33,34,29,30],"tags":[],"class_list":["post-2795","post","type-post","status-publish","format-standard","hentry","category-do-biology","category-lets-do-chemistry","category-lets-do-science","category-recent-science-news"],"aioseo_notices":[],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack-related-posts":[{"id":2523,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2523","url_meta":{"origin":2795,"position":0},"title":"Cryptic DNA sequences may help cells survive starvation","author":"biochemistry","date":"January 18, 2019","format":false,"excerpt":"\u00a0 \u00a0 Stretches of non-coding DNA in genes called introns could have an important survival function. \u00a0 Non-coding 'intron' DNA can help yeast cells survive at times of stress.Credit: Michael Abbey\/Science Photo Library Patches of seemingly meaningless DNA dotted throughout the genome might actually have a function: helping cells to\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":2582,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2582","url_meta":{"origin":2795,"position":1},"title":"Technologies to watch in 2019","author":"biochemistry","date":"January 29, 2019","format":false,"excerpt":"\u00a0 \u00a0 From higher-resolution imaging to genome-sized DNA molecules built from scratch, the year ahead looks exciting for life-science technology. \u00a0 An automated bioreactor system for growing yeast, which can be used to investigate synthetic genomes \u2014 one area poised to make big strides this year.Credit: Tim Llewellyn\/Ginkgo Bioworks \u00a0\u2026","rel":"","context":"In &quot;Essays on Science&quot;","block_context":{"text":"Essays on Science","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=32"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":3202,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=3202","url_meta":{"origin":2795,"position":2},"title":"DNA sequencing at 40: past, present and future","author":"biochemistry","date":"April 5, 2019","format":false,"excerpt":"\u00a0 \u00a0 Abstract \u00a0 This review commemorates the 40th anniversary of DNA sequencing, a period in which we have already witnessed multiple technological revolutions and a growth in scale from a few kilobases to the first human genome, and now to millions of human and a myriad of other genomes.\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":4845,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=4845","url_meta":{"origin":2795,"position":3},"title":"CRISPR tool modifies genes precisely by copying RNA into the genome &#038; CRISPR: the movie","author":"biochemistry","date":"November 15, 2019","format":false,"excerpt":"\u00a0 \u00a0 The ultimate goal of genome editing is to be able to make any specific change to the blueprint of life. A \u2018search-and-replace\u2019 method for genome editing takes us a giant leap closer to this ambitious goal. \u00a0 \u00a0 Variation in the DNA sequences that constitute the blueprint of\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":2993,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2993","url_meta":{"origin":2795,"position":4},"title":"Gene expression at fine scale","author":"biochemistry","date":"March 29, 2019","format":false,"excerpt":"\u00a0 \u00a0 Mapping gene expression at the single-cell level within tissues remains a technical challenge. Rodriques\u00a0et al.\u00a0developed a method called Slide-seq, whereby RNA was spatially resolved from tissue sections by transfer onto a surface covered with DNA-barcoded beads. Applying Slide-seq to regions of a mouse brain revealed spatial gene expression\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":4207,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=4207","url_meta":{"origin":2795,"position":5},"title":"The structure of DNA","author":"biochemistry","date":"October 11, 2019","format":false,"excerpt":"\u00a0 \u00a0 In the early 1950s, the identity of genetic material was still a matter of debate. The discovery of the helical structure of double-stranded DNA settled the matter \u2014 and changed biology forever. \u00a0 \u00a0 On 25 April 1953, James Watson and Francis Crick announced1\u00a0in\u00a0Nature\u00a0that they \u201cwish to suggest\u201d\u00a0a\u2026","rel":"","context":"In &quot;Essays on Science&quot;","block_context":{"text":"Essays on Science","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=32"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]}],"jetpack_sharing_enabled":false,"jetpack_shortlink":"https:\/\/wp.me\/p9Xo1j-J5","_links":{"self":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts\/2795","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2795"}],"version-history":[{"count":1,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts\/2795\/revisions"}],"predecessor-version":[{"id":2796,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts\/2795\/revisions\/2796"}],"wp:attachment":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2795"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2795"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2795"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}