{"id":2718,"date":"2019-02-22T12:46:27","date_gmt":"2019-02-22T03:46:27","guid":{"rendered":"http:\/\/163.180.4.222\/lab\/?p=2718"},"modified":"2019-02-22T12:46:27","modified_gmt":"2019-02-22T03:46:27","slug":"a-single-cell-molecular-map-of-mouse-gastrulation-and-early-organogenesis","status":"publish","type":"post","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2718","title":{"rendered":"A single-cell molecular map of mouse gastrulation and early organogenesis"},"content":{"rendered":"<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h5 class=\"js-section-title section-title strong position-relative tighten-line-height background-gray-light pt20 pb6 pl0 pr20 standard-space-below small-space-above mq640-pt10 mq640-pb10 mq640-pl20 mq640-mt0 mq640-ml-20 mq640-mr-20 extend-left\">Abstract<\/h5>\n<p>&nbsp;<\/p>\n<div id=\"Abs1-content\" class=\"pl20 mq875-pl0 js-collapsible-section\">\n<p>Across the animal kingdom, gastrulation represents a key developmental event during which embryonic pluripotent cells diversify into lineage-specific precursors that will generate the adult organism. Here we report the transcriptional profiles of 116,312 single cells from mouse embryos collected at nine sequential time points ranging from 6.5 to 8.5\u00a0days post-fertilization. We construct a molecular map of cellular differentiation from pluripotency towards all major embryonic lineages, and explore the complex events involved in the convergence of visceral and primitive streak-derived endoderm. Furthermore, we use single-cell profiling to show that\u00a0<i>Tal1<\/i><sup>\u2212\/\u2212<\/sup>\u00a0chimeric embryos display defects in early mesoderm diversification, and we thus demonstrate how combining temporal and transcriptional information can illuminate gene function. Together, this comprehensive delineation of mammalian cell differentiation trajectories in vivo represents a baseline for understanding the effects of gene mutations during development, as well as a roadmap for the optimization of in vitro differentiation protocols for regenerative medicine.<\/p>\n<\/div>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>(\uc6d0\ubb38: <a href=\"https:\/\/www.nature.com\/articles\/s41586-019-0933-9?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29\">\uc5ec\uae30<\/a>\ub97c \ud074\ub9ad\ud558\uc138\uc694~)<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>&nbsp; &nbsp; Abstract &nbsp; Across the animal kingdom, gastrulation represents a key developmental event during which embryonic pluripotent cells diversify into lineage-specific precursors that will<a href=\"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2718\" class=\"more-link\">(more&#8230;)<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[33,29,30],"tags":[],"class_list":["post-2718","post","type-post","status-publish","format-standard","hentry","category-do-biology","category-lets-do-science","category-recent-science-news"],"aioseo_notices":[],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack-related-posts":[{"id":4849,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=4849","url_meta":{"origin":2718,"position":0},"title":"Modeling the early development of a primate embryo","author":"biochemistry","date":"November 15, 2019","format":false,"excerpt":"\u00a0 \u00a0 Because mammalian embryos develop inside the uterus after implantation, they are practically inaccessible for direct observation and experimental analysis of the developmental process. To visualize and study the development of post-implantation embryos, it is necessary to develop a technology that maintains the viability and growth of embryos ex\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":2016,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2016","url_meta":{"origin":2718,"position":1},"title":"How to lose your inheritance","author":"biochemistry","date":"October 5, 2018","format":false,"excerpt":"\u00a0 \u00a0 \ubc30\uc544\uc904\uae30\uc138\ud3ec\uc758 \ubd84\ud654 \uacfc\uc815\uc5d0\uc11c\uc758 \uc804\uc0ac\uc778\uc790\uc758 \uc911\uc694\uc131\uc5d0 \uad00\ud55c \ub0b4\uc6a9\uc785\ub2c8\ub2e4. (\uc6d0\ubb38: \uc5ec\uae30\ub97c \ud074\ub9ad\ud558\uc138\uc694~) \u00a0 In developing embryos, molecular and physical differences divide the cells that will form eggs or sperm and those that will form the body. The mouse protein OTX2 directs this decision by blocking reproductive-cell fate. \u00a0 \u00a0 In\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":1363,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=1363","url_meta":{"origin":2718,"position":2},"title":"CRISPR \u2018barcodes\u2019 map mammalian development in exquisite detail","author":"biochemistry","date":"August 14, 2018","format":false,"excerpt":"\u00a0 \u00a0 (\uc6d0\ubb38) \u00a0 \u00a0 Genome-editing technique enables researchers to trace lineage of cells in developing mice. \u00a0 \u00a0 Researchers have used gene-editing to track the cell-by-cell development of a mouse embryo.\u00a0Credit: Agnieszka Jedrusik and Magdalena Zernicka-Goetz, Gurdon Institute.\u00a0CC0 \u00a0 \u00a0 For the first time, scientists have wielded CRISPR to\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":4717,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=4717","url_meta":{"origin":2718,"position":3},"title":"Primate embryos grown in the lab for longer than ever before","author":"biochemistry","date":"November 2, 2019","format":false,"excerpt":"\u00a0 \u00a0 The 20-day-old monkey embryos could reopen the debate about how long the human variety should be allowed to grow in a dish. \u00a0 \u00a0 Two groups have grown cynomolgus monkey embryos for 20 days in the lab.Credit: Mark MacEwen\/Nature Picture Library \u00a0 \u00a0 They are the longest lived\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":3734,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=3734","url_meta":{"origin":2718,"position":4},"title":"Cell fate decisions during development","author":"biochemistry","date":"June 8, 2019","format":false,"excerpt":"\u00a0 \u00a0 The shape of our nose, the color of our skin, the movement of our gut, all depend on an extraordinary cell type called neural crest cells, which originate during embryogenesis. Since their discovery in 1868 (1), neural crest cells, which are present in all vertebrates, have fascinated developmental\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":4472,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=4472","url_meta":{"origin":2718,"position":5},"title":"Cell identity reprogrammed","author":"biochemistry","date":"October 16, 2019","format":false,"excerpt":"\u00a0 \u00a0 The discovery that cell differentiation can be reversed challenged theories of how cell identity is determined, laying the foundations for modern methods of reprogramming cell identity and promising new regenerative therapies. \u00a0 \u00a0 All cells of an organism derive from a single cell. As development progresses, cells become\u2026","rel":"","context":"In &quot;'10. \uac1c\uccb4\uc758 \uc815\uccb4\uc131\uacfc \uac1c\uccb4 \uac04 \uc0c1\ud638\uc791\uc6a9'\uacfc '11. \uc9c4\ud654\uc758 \uba54\ucee4\ub2c8\uc998' \uad00\ub828&quot;","block_context":{"text":"'10. \uac1c\uccb4\uc758 \uc815\uccb4\uc131\uacfc \uac1c\uccb4 \uac04 \uc0c1\ud638\uc791\uc6a9'\uacfc '11. \uc9c4\ud654\uc758 \uba54\ucee4\ub2c8\uc998' \uad00\ub828","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=44"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]}],"jetpack_sharing_enabled":false,"jetpack_shortlink":"https:\/\/wp.me\/p9Xo1j-HQ","_links":{"self":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts\/2718","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2718"}],"version-history":[{"count":1,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts\/2718\/revisions"}],"predecessor-version":[{"id":2719,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts\/2718\/revisions\/2719"}],"wp:attachment":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2718"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2718"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2718"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}