{"id":2675,"date":"2019-02-19T15:19:27","date_gmt":"2019-02-19T06:19:27","guid":{"rendered":"http:\/\/163.180.4.222\/lab\/?p=2675"},"modified":"2019-02-19T15:19:27","modified_gmt":"2019-02-19T06:19:27","slug":"multi-omic-measurements-of-heterogeneity-in-hela-cells-across-laboratories","status":"publish","type":"post","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2675","title":{"rendered":"Multi-omic measurements of heterogeneity in HeLa cells across laboratories"},"content":{"rendered":"<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h4 id=\"Abs1\" class=\"js-section-title section-title strong position-relative tighten-line-height background-gray-light pt20 pb6 pl0 pr20 standard-space-below small-space-above mq640-pt10 mq640-pb10 mq640-pl20 mq640-mt0 mq640-ml-20 mq640-mr-20 extend-left\">Abstract<\/h4>\n<div id=\"Abs1-content\" class=\"pl20 mq875-pl0 js-collapsible-section\">\n<p>&nbsp;<\/p>\n<p>Reproducibility in research can be compromised by both biological and technical variation, but most of the focus is on removing the latter. Here we investigate the effects of biological variation in HeLa cell lines using a systems-wide approach. We determine the degree of molecular and phenotypic variability across 14 stock HeLa samples from 13 international laboratories. We cultured cells in uniform conditions and profiled genome-wide copy numbers, mRNAs, proteins and protein turnover rates in each cell line. We discovered substantial heterogeneity between HeLa variants, especially between lines of the CCL2 and Kyoto varieties, and observed progressive divergence within a specific cell line over 50 successive passages. Genomic variability has a complex, nonlinear effect on transcriptome, proteome and protein turnover profiles, and proteotype patterns explain the varying phenotypic response of different cell lines to\u00a0<i>Salmonella<\/i>\u00a0infection. These findings have implications for the interpretation and reproducibility of research results obtained from human cultured cells.<\/p>\n<\/div>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>(\uc6d0\ubb38: <a href=\"https:\/\/www.nature.com\/articles\/s41587-019-0037-y?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nbt%2Frss%2Fcurrent+%28Nature+Biotechnology+-+Issue%29\">\uc5ec\uae30<\/a>\ub97c \ud074\ub9ad\ud558\uc138\uc694~)<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>&nbsp; &nbsp; Abstract &nbsp; Reproducibility in research can be compromised by both biological and technical variation, but most of the focus is on removing the<a href=\"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2675\" class=\"more-link\">(more&#8230;)<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[33,34,29,30],"tags":[],"class_list":["post-2675","post","type-post","status-publish","format-standard","hentry","category-do-biology","category-lets-do-chemistry","category-lets-do-science","category-recent-science-news"],"aioseo_notices":[],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack-related-posts":[{"id":3881,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=3881","url_meta":{"origin":2675,"position":0},"title":"Caging cells in functional shells","author":"biochemistry","date":"July 16, 2019","format":false,"excerpt":"\u00a0 \u00a0 Unprotected cells are sensitive to changes in their environment, including temperature, pH, and osmotic pressure. Zhu\u00a0et al.\u00a0have developed a facile route to encapsulating mammalian cells inside functional nanoparticles. A range of nanoparticles, encompassing several types of metal-organic frameworks, mesoporous silica, and iron oxide, were mixed with interparticle ligands\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":3470,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=3470","url_meta":{"origin":2675,"position":1},"title":"The new techniques revealing the varied shapes of chromatin","author":"biochemistry","date":"May 7, 2019","format":false,"excerpt":"\u00a0 \u00a0 Researchers are realizing that the DNA\u2013protein complex doesn\u2019t just have one form but many. \u00a0 This multicoloured image of chromatin was created using multiplexed fluorescence\u00a0in situ\u00a0hybridization and super-resolution microscopy.Credit: Bogdan Bintu\/The Xiaowei Zhuang Laboratory\/The Alistair Boettiger Laboratory \u00a0 \u00a0 Molecular models suggest that chromosomes assemble in an ordered,\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":1859,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=1859","url_meta":{"origin":2675,"position":2},"title":"Programmable protein circuits in living cells","author":"biochemistry","date":"September 25, 2018","format":false,"excerpt":"\u00a0 \u00a0 Science\u00a0\u00a021 Sep 2018: Vol. 361, Issue 6408, pp. 1252-1258 DOI: 10.1126\/science.aat5062 \u00a0 \uc5ec\uae30\ub97c \ud074\ub9ad\ud558\uc138\uc694~ \u00a0 \u00a0 Building smarter synthetic biological circuits Synthetic genetic and biological regulatory circuits can enable logic functions to form the basis of biological computing; synthetic biology can also be used to control cell behaviors\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":4114,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=4114","url_meta":{"origin":2675,"position":3},"title":"Glowing DNA label illuminates a cell\u2019s fine details","author":"biochemistry","date":"September 23, 2019","format":false,"excerpt":"\u00a0 \u00a0 Fluorescent tag can be affixed to proteins or genetic structures of interest. \u00a0 \u00a0 DNA tags could help scientists to study ion channels (yellow), which allow specific ions to pass through the membrane of nerve cells (green). Credit: Patrick Landmann\/SPL \u00a0 \u00a0 \u00a0 A glowing tag made of\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":4784,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=4784","url_meta":{"origin":2675,"position":4},"title":"Beyond the genome: RNA control of stem cells","author":"biochemistry","date":"November 8, 2019","format":false,"excerpt":"\u00a0 \u00a0 Tissue-resident stem cells are important for maintaining proper organ and tissue function throughout the lifetime of mammals. Although some types of stem cells constantly proliferate and give rise to committed progeny, such as intestine and skin, others reside mainly in a quiescent (noncycling) state, such as skeletal muscle.\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":3489,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=3489","url_meta":{"origin":2675,"position":5},"title":"Biosimilars: mimicking biological drugs","author":"biochemistry","date":"May 9, 2019","format":false,"excerpt":"\u00a0 \u00a0 With the patents on many biological drugs soon to expire, the biosimilars revolution is about to shift into top gear. \u00a0 Credit: Andrew Khosravani \u00a0 \u00a0 Biological drugs (biologics) are a crucial component of the pharmaceutical arsenal. This class of drug is typically manufactured through engineered biological processes\u2026","rel":"","context":"In &quot;Essays on Science&quot;","block_context":{"text":"Essays on Science","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=32"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]}],"jetpack_sharing_enabled":false,"jetpack_shortlink":"https:\/\/wp.me\/p9Xo1j-H9","_links":{"self":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts\/2675","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2675"}],"version-history":[{"count":1,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts\/2675\/revisions"}],"predecessor-version":[{"id":2676,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts\/2675\/revisions\/2676"}],"wp:attachment":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2675"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2675"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2675"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}