{"id":2590,"date":"2019-01-30T12:47:34","date_gmt":"2019-01-30T03:47:34","guid":{"rendered":"http:\/\/163.180.4.222\/lab\/?p=2590"},"modified":"2019-02-08T11:53:44","modified_gmt":"2019-02-08T02:53:44","slug":"dna-%ed%8e%b8%ec%a7%91%ec%9d%84-%ec%9c%84%ed%95%9c-%ec%83%88-%eb%8f%84%ea%b5%ac","status":"publish","type":"post","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2590","title":{"rendered":"DNA \ud3b8\uc9d1\uc744 \uc704\ud55c \uc0c8 \ub3c4\uad6c"},"content":{"rendered":"

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[<\/span>\uc694\uc57d]<\/span>\ubbf8\uad6d\u00a0MIT<\/span>\uc640 \ud558\ubc84\ub4dc\ub300 \uacf5\ub3d9\uc5f0\uad6c\ud300\uc774 \uc720\uc804\uc790 \uac00\uc704\uc778 \ud06c\ub9ac\uc2a4\ud37c\uc5d0 \ud65c\uc6a9\ud560 \uc218 \uc788\ub294 \uc0c8\ub85c\uc6b4 \uae30\uc220\uc744 \uc644\uc131\ud588\ub2e4.\u00a0\uc774 \ud06c\ub9ac\uc2a4\ud37c \uc720\uc804\uc790 \uac00\uc704\ub294 \ud604\uc7ac \ub110\ub9ac \ud65c\uc6a9\ub418\ub294 \ub3c4\uad6c \uc138\ud2b8\uc640\u00a0\ube44\uad50\ud560 \ub54c \ud5a5\uc0c1\ub41c \uae30\ub2a5\uacfc \ub2e4\uc591\ud55c \uc635\uc158\uc744 \uc81c\uacf5\ud55c\ub2e4.(2019.01)
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\/MIT : Cas12b\uc758 \uacb0\uc815 \uad6c\uc870. \uae08\uc0c9\uc740 RNA, \uac08\uc0c9\uc740 DNA\uc774\ub2e4.<\/span><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n
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\ubbf8\uad6d \uad6d\ub9bd\ubcf4\uac74\uc6d0(NIH)\uc758\u00a0\uc720\uc9c4 \ucfe0\ub2cc\uacfc\u00a0MIT\u00a0\ube0c\ub85c\ub4dc \uc778\uc2a4\ud2f0\ud29c\ud2b8,\u00a0\ud558\ubc84\ub4dc\ub300 \ud3ad\uc7a5 \uad50\uc218 \uacf5\ub3d9 \uc5f0\uad6c\ud300\uc740 \uad6d\uc81c\ud559\uc220\uc9c0\u00a0\u2018\ub124\uc774\ucc98 \ucee4\ubba4\ub2c8\ucf00\uc774\uc158\uc2a4\u2019\u00a0\ucd5c\uc2e0\ud638\uc5d0 \uac8c\uc7ac\ud55c \ub17c\ubb38\uc5d0\uc11c \uc778\uac04 \uc138\ud3ec \ud3b8\uc9d1\uc5d0 \ucd5c\uc801\ud654\ub41c \uc0c8\ub85c\uc6b4 \ud6a8\uc18c\ub97c\u00a0\ud65c\uc6a9\ud55c \ub3c4\uad6c \uc138\ud2b8\ub97c\u00a0\ucc3e\uc544\ub0c8\ub2e4\uace0 \ubc1d\ud614\ub2e4. Bacillus hisashii (BhCas12b)\ub77c\ub294 \uc774 \ud6a8\uc18c\ub294 \ud604\uc7ac \ub110\ub9ac \ud65c\uc6a9\ub418\ub294\u00a0Cas9\uc5d0 \ube44\ud574 \ud06c\uae30\uac00 \uc791\uace0 \ud45c\uc801\uc744 \ub354 \uc798 \ucc3e\uc544\ub0b8\ub2e4.<\/span><\/p>\n

\uc5f0\uad6c\ud300\uc740\u00a02015\ub144 \uc774\ubbf8\u00a0Cas12b\ub97c \ud06c\ub9ac\uc2a4\ud3ec \uc720\uc804\uc790 \uac00\uc704\uc5d0 \uc0ac\uc6a9\ud560 \uc218 \uc788\ub294 \uc0c8\ub85c\uc6b4 \uc720\ub825 \ud6a8\uc18c\u00a03\uac00\uc9c0 \uc911 \ud558\ub098\ub85c \uaf3d\uc558\ub2e4.\u00a0\ud558\uc9c0\ub9cc \uacb0\uc815\uc801\uc778 \ubb38\uc81c\uac00 \uc788\uc5c8\ub2e4. Cas12b\ub294 \uac04\ud5d0\ucc9c,\u00a0\uc628\ucc9c,\u00a0\ud654\uc0b0 \uae4a\uc740 \uacf3\uc758 \ub728\uac70\uc6b4 \ud658\uacbd\uc5d0 \uc0ac\ub294 \uace0\uc628\uc131 \ubc15\ud14c\ub9ac\uc544\uc5d0\uc11c \uc5bb\uc5b4\uc9c4\ub2e4.\u00a0\uc774\ub294 \uc774 \ud6a8\uc18c\uac00 \uc790\uc5f0\uc801\uc73c\ub85c\ub294 \uc0ac\ub78c\uc758 \uccb4\uc628\ubcf4\ub2e4 \ub192\uc740 \uc628\ub3c4\uc5d0\uc11c\ub9cc \ud65c\ub3d9\ud55c\ub2e4\ub294 \ub73b\uc774\ub2e4.<\/p>\n

\ud3ad\ucc3d \uad50\uc218\ub294\u00a0\u201c\uc6b0\ub9ac\ub294 \uc800\uc628\uc5d0\uc11c \uc791\ub3d9\ud560 \uc218 \uc788\ub294\u00a0Cas12b\u00a0\ubc84\uc804\uc744 \ub9cc\ub4e4\uace0 \uc2f6\uc5c8\ub2e4\u201d\uba74\uc11c\u00a0\u201c\uc218\ucc9c\uac1c\uc758 \ubc15\ud14c\ub9ac\uc544 \uc720\uc804\uccb4 \uc11c\uc5f4\uc744 \ubd84\uc11d\ud574 \ud3ec\uc720\ub958\uc758 \uccb4\uc628\uacfc \uac19\uc740 \ub0ae\uc740 \uc628\ub3c4\uc5d0\uc11c \ud65c\ub3d9\ud558\ub294 \ubc15\ud14c\ub9ac\uc544\ub97c \ucc3e\uc558\ub2e4\u201d\uace0 \ub9d0\ud588\ub2e4.\u00a0\uc720\ub9dd\ud55c \ud6c4\ubcf4 \ud6a8\uc18c\ub4e4\uc744 \uc870\ud569\ud558\uba74\uc11c \uc5f0\uad6c\ud300\uc740 \uc778\uac04 \uba74\uc5ed\uc138\ud3ec(T\uc138\ud3ec)\uc758 \uac8c\ub188\uc744 \ud6a8\uc728\uc801\uc73c\ub85c \ud3b8\uc9d1\ud560 \uc218 \uc788\ub294\u00a0Cas12b\ub97c \ub9cc\ub4e4\uc5b4\ub0c8\ub2e4.\u00a0\uc7a5\uae30\uc801\uc73c\ub85c \uba74\uc5ed\uad00\ub828 \uc9c8\ud658 \uce58\ub8cc\uc81c\ub97c \uc720\uc804\uc790 \ud3b8\uc9d1\uc73c\ub85c \ub9cc\ub4e4\uc5b4\ub0bc \uac00\ub2a5\uc131\uc774 \uc5f4\ub9b0 \uac83\uc774\ub2e4.<\/p>\n

\ub17c\ubb38\uc758\u00a01\uc800\uc790\uc778 \uc870\ub098\ub2e8 \uc2a4\ud2b8\ub808\ucee4\ub294\u00a0\u201c\uc774\ub294 \uc55e\uc73c\ub85c\ub3c4 \uc720\uc6a9\ud55c \ud06c\ub9ac\uc2a4\ud37c \uc2dc\uc2a4\ud15c\uc774 \uc5bc\ub9c8\ub4e0\uc9c0 \ub9ce\uc774 \ubc1c\uacac\ub420 \uc218 \uc788\ub2e4\ub294 \uc99d\uac70\u201d\ub77c\uace0 \ub9d0\ud588\ub2e4.\u00a0\ud06c\ub9ac\uc2a4\ud37c \uc720\uc804\uc790 \uac00\uc704 \uc5f0\uad6c\ub294 \ube60\ub978 \uc18d\ub3c4\ub85c \ubc1c\uc804\ud558\uace0 \uc788\ub2e4. 2017\ub144\u00a0UC\ubc84\ud074\ub9ac \uc5f0\uad6c\ud300\uc740\u00a0Cas12b\uac00 \uc2dc\ud5d8\uad00\ub0b4\uc5d0\uc11c\u00a0DNA\uc758 \ube44\ud2b9\uc774\uc801 \ub2f4\uccb4 \uc808\ub2e8\uc5d0 \ud65c\uc6a9\ub420 \uc218 \uc788\ub2e4\ub294 \uc5f0\uad6c \uacb0\uacfc\ub97c \ub0b4\ub193\uc558\ub2e4.\u00a0\ucd5c\uadfc \uc911\uad6d \ubca0\uc774\uc9d5\uc758 \uc911\uad6d\uacfc\ud559\uc544\uce74\ub370\ubbf8 \uc5f0\uad6c\ud300\uc740 \ubbf8\uc0dd\ubb3c\uc758 \uc77c\uc885\uc778\u00a0Alicyclobacillus acidiphilus\uc5d0\uc11c \uc5bb\uc740\u00a0Cas12b\uac00 \ud3ec\uc720 \ub3d9\ubb3c \uc138\ud3ec \ud3b8\uc9d1\uc5d0 \uc0ac\uc6a9\ub410\ub2e4\uace0 \ubcf4\uace0\ud558\uae30\ub3c4 \ud588\ub2e4.<\/p>\n

\ube0c\ub85c\ub4dc \uc778\uc2a4\ud2f0\ud29c\ud2b8\uc640\u00a0MIT\ub294 \uc774\uc804 \uac8c\ub188 \ud3b8\uc9d1 \ub3c4\uad6c\uc640 \ub9c8\ucc2c\uac00\uc9c0\ub85c \uc774\ubc88\uc5d0 \uac1c\ubc1c\ud55c\u00a0Cas12b\u00a0\uc2dc\uc2a4\ud15c\uc744 \ub110\ub9ac \uacf5\uc720\ud560 \uc608\uc815\uc774\ub2e4.\u00a0\uc5f0\uad6c\ud300\uc740 \ud06c\ub9ac\uc2a4\ud37c \uc720\uc804\uc790 \uac00\uc704 \uc5f0\uad6c\ub97c \uc804\uc138\uacc4\uc801\uc73c\ub85c \uac00\uc18d\ud558\uae30 \uc704\ud574 \uc5f0\uad6c\uc2e4 \ud648\ud398\uc774\uc9c0\uc5d0\uc11c \uc5bc\ub9c8\ub4e0\uc9c0 \uad00\ub828 \uc815\ubcf4\ub97c \uc5bb\ub3c4\ub85d \ud558\uace0 \uc788\ub2e4.\u00a0\ud604\uc7ac \uc804\uc138\uacc4\u00a062\uac1c\uad6d\u00a02400\uc5ec\uac1c \uc5f0\uad6c\uc2e4\uc5d0\uc11c \uc774 \uc815\ubcf4\ub97c \ud65c\uc6a9\ud574 \uc5f0\uad6c\ub97c \uc9c4\ud589\ud558\uace0 \uc788\ub2e4.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

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(\uc6d0\ubb38: \uc5ec\uae30<\/a>\ub97c \ud074\ub9ad\ud558\uc138\uc694~)<\/p>\n

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CasX enzymes comprise a distinct family of RNA-guided genome editors<\/h4>\n

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Abstract<\/h5>\n
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The RNA-guided CRISPR-associated (Cas) proteins Cas9 and Cas12a provide adaptive immunity against invading nucleic acids, and function as powerful tools for genome editing in a wide range of organisms. Here we reveal the underlying mechanisms of a third, fundamentally distinct RNA-guided genome-editing platform named CRISPR\u2013CasX, which uses unique structures for programmable double-stranded DNA\u00a0binding and cleavage. Biochemical and in vivo data demonstrate that CasX is active for\u00a0Escherichia coli<\/i>\u00a0and human genome modification. Eight cryo-electron microscopy structures of CasX in different states of assembly with its guide RNA and double-stranded DNA substrates reveal an extensive RNA scaffold and a domain required for DNA unwinding. These data demonstrate how CasX activity arose through convergent evolution to establish an enzyme family that is functionally separate from both Cas9 and Cas12a.<\/p>\n<\/div>\n

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    [\uc694\uc57d]\ubbf8\uad6d\u00a0MIT\uc640 \ud558\ubc84\ub4dc\ub300 \uacf5\ub3d9\uc5f0\uad6c\ud300\uc774 \uc720\uc804\uc790 \uac00\uc704\uc778 \ud06c\ub9ac\uc2a4\ud37c\uc5d0 \ud65c\uc6a9\ud560 \uc218 \uc788\ub294 \uc0c8\ub85c\uc6b4 \uae30\uc220\uc744 \uc644\uc131\ud588\ub2e4.\u00a0\uc774 \ud06c\ub9ac\uc2a4\ud37c \uc720\uc804\uc790 \uac00\uc704\ub294 \ud604\uc7ac \ub110\ub9ac \ud65c\uc6a9\ub418\ub294 \ub3c4\uad6c \uc138\ud2b8\uc640\u00a0\ube44\uad50\ud560 \ub54c \ud5a5\uc0c1\ub41c \uae30\ub2a5\uacfc(more…)<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[33,34,29,30],"tags":[],"class_list":["post-2590","post","type-post","status-publish","format-standard","hentry","category-do-biology","category-lets-do-chemistry","category-lets-do-science","category-recent-science-news"],"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t