{"id":2580,"date":"2019-01-29T16:00:33","date_gmt":"2019-01-29T07:00:33","guid":{"rendered":"http:\/\/163.180.4.222\/lab\/?p=2580"},"modified":"2019-01-29T16:00:33","modified_gmt":"2019-01-29T07:00:33","slug":"on-the-road-to-a-gene-drive-in-mammals","status":"publish","type":"post","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2580","title":{"rendered":"On the road to a gene drive in mammals"},"content":{"rendered":"<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>A method for making a version of a gene more likely to be inherited than normal, generating what is called a gene drive, might be used to control insect populations. It has now been reported to work in mammals, too.<\/p>\n<p>&nbsp;<\/p>\n<div class=\"article__body serif cleared\">\n<p>When Gregor Mendel tracked pea-plant characteristics over successive generations in the nineteenth century<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-00185-y?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR1\">1<\/a><\/sup>, his landmark study revealed key insights into the fundamental mechanisms governing genetic inheritance. Mendel observed consistent patterns of inheritance that corresponded to each descendant receiving one of the two maternal copies of a gene affecting the characteristic and one of the two paternal copies of this gene. In this typical scenario of genetic inheritance, both maternal copies of a gene have an equal probability of being inherited, as do both paternal copies.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<aside class=\"recommended pull pull--left sans-serif\" data-label=\"Related\"><a href=\"https:\/\/www.nature.com\/articles\/s41586-019-0875-2\" data-track=\"click\" data-track-label=\"recommended article\"><img decoding=\"async\" class=\"recommended__image\" src=\"https:\/\/media.nature.com\/w400\/magazine-assets\/d41586-019-00185-y\/d41586-019-00185-y_16405290.jpg\" \/><\/a><\/p>\n<p class=\"recommended__title serif\">Read the paper: Super-Mendelian inheritance mediated by CRISPR\u2013Cas9 in the female mouse germline<\/p>\n<\/aside>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>However, inheritance does not always proceed so fairly, and in some cases the odds of a particular copy of a gene being transmitted to the next generation can be heavily skewed. One natural example is that of \u2018jumping genes\u2019, which are inherited in a non-Mendelian pattern<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-00185-y?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR2\">2<\/a><\/sup><i>.\u00a0<\/i>Genetic-engineering approaches are being developed to manipulate the inheritance pattern of a gene copy such that it will spread through a population more rapidly than would be expected by normal Mendelian inheritance, generating what is called a gene drive and leading to super-Mendelian inheritance<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-00185-y?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR3\">3<\/a><\/sup><sup>,<\/sup><sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-00185-y?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR4\">4<\/a><\/sup>. This process generates what is called a gene drive. So far, gene drives have been mainly engineered in insects.\u00a0<a href=\"https:\/\/www.nature.com\/articles\/s41586-019-0875-2\" data-track=\"click\" data-label=\"https:\/\/www.nature.com\/articles\/s41586-019-0875-2\" data-track-category=\"body text link\">Writing in\u00a0<i>Nature<\/i><\/a>, Grunwald\u00a0<i>et al<\/i>.<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-00185-y?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR5\">5<\/a><\/sup>\u00a0report a method for generating a gene drive in mice, offering an option to use this approach in mammals.<\/p>\n<p>Gene drives developed in insects might provide a way to alter mosquito populations to decrease the probability that they transmit diseases such as malaria or dengue fever<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-00185-y?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR3\">3<\/a><\/sup><sup>,<\/sup><sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-00185-y?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR4\">4<\/a><\/sup>. For example, a gene drive that affects mosquito fertility could be used to specifically eliminate a species of malaria-transmitting mosquito<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-00185-y?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR4\">4<\/a><\/sup>, allowing its ecological niche to be filled by other mosquito species that cannot harbour the malaria-causing parasite. Alternatively, gene drives can be designed<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-00185-y?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR6\">6<\/a><\/sup>\u00a0to confer widespread, species-specific resistance to infection by this parasite, for instance by using a gene drive to spread sequences that encode antimalarial antibodies so that mosquitoes are no longer infected by the parasite<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-00185-y?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR7\">7<\/a><\/sup>.<\/p>\n<p>The technology needed for gene drives has been greatly accelerated in insects by harnessing a gene-editing technique called CRISPR<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-00185-y?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR3\">3<\/a><\/sup><sup>,<\/sup><sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-00185-y?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR4\">4<\/a><\/sup><sup>,<\/sup><sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-00185-y?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR6\">6<\/a><\/sup>. This system relies on the insects being engineered to express the enzyme Cas9 and a guide RNA that provides gene-targeting specificity. Cas9 generates a cut in a genomic DNA sequence that matches the guide RNA sequence (Fig. 1). If the guided cut generates a double-stranded DNA break in one copy of a gene, this break can be repaired by a process called homologous recombination, in which the undamaged chromosome containing a sequence that matches that in the region of the DNA break is used as a repair template.<\/p>\n<figure class=\"figure\">\n<div class=\"embed intensity--high\">\n<div class=\"embed intensity--high\"><img decoding=\"async\" class=\"figure__image\" src=\"https:\/\/media.nature.com\/w800\/magazine-assets\/d41586-019-00185-y\/d41586-019-00185-y_16408418.jpg\" alt=\"\" data-src=\"\/\/media.nature.com\/w800\/magazine-assets\/d41586-019-00185-y\/d41586-019-00185-y_16408418.jpg\" \/><\/div>\n<\/div><figcaption>\n<p class=\"figure__caption sans-serif\"><span class=\"mr10\"><b>Figure 1 | Engineering super-Mendelian inheritance in mice.<\/b>\u00a0<b>a<\/b>, If mice have a mutation in both copies of their\u00a0<i>Tyr<\/i>\u00a0gene, their coat is white, but if one copy of\u00a0<i>Tyr<\/i>\u00a0is functional, their coat is grey. Coat colour can therefore be used to track the inheritance of versions of this gene. In the normal pattern of genetic inheritance, termed Mendelian inheritance, both parental copies of a gene have an equal probability of being included in reproductive cells called germ cells, which are passed to the next generation. Therefore, in a cross between a parent that has two mutant copies of\u00a0<i>Tyr<\/i>\u00a0and a parent with one mutant copy and one wild-type copy, the predicted Mendelian inheritance pattern is that half the offspring will have two mutant copies of\u00a0<i>Tyr<\/i>\u00a0and half will have one mutant and one wild-type copy.\u00a0<b>b<\/b>, Grunwald\u00a0<i>et al<\/i>.<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-00185-y?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR5\">5<\/a><\/sup>\u00a0used a genetic-engineering approach termed CRISPR to alter the pattern of genetic inheritance in mice, generating what is called a gene drive. They inserted a sequence called a CopyCat cassette into\u00a0<i>Tyr<\/i>\u00a0at a location that prevents\u00a0<i>Tyr<\/i>\u00a0from encoding a functional protein. This cassette encodes a CRISPR component called a guide RNA that enables another CRISPR component, the protein Cas9, to specifically cut the wild-type copy of\u00a0<i>Tyr<\/i>. The authors found that, in the reproductive tissues of female mice, such a cut is repaired by a process called homologous recombination (HR), which uses the copy of\u00a0<i>Tyr<\/i>\u00a0containing the CopyCat cassette as a template, and so results in a cell that contains two copies of\u00a0<i>Tyr<\/i>\u00a0that have this cassette.\u00a0<b>c<\/b>, The authors observed that using such a Cas9-mediated gene drive resulted in more than half of the germ cells containing the CopyCat cassette, rather than the 50% expected. This causes a manipulated pattern of gene inheritance, termed super-Mendelian.<\/span><\/p>\n<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>A DNA sequence needed for the gene drive, called a cassette, which encodes CRISPR machinery, can be engineered and inserted into a chosen site in a host chromosome. The cassette encodes components needed to initiate a targeted Cas9-mediated DNA break on the sister chromosome. Successful repair of this break by homologous recombination using the chromosome that contains the cassette results in both the maternal and paternal sister chromosomes having identical copies of this cassette (a state called homozygosity). The cassette can be engineered to deliver additional DNA sequences, and such gene editing results in cells that are homozygous for any desired gene on the cassette. Achieving this effect consistently in the reproductive cells (germ cells) would ensure that all offspring receive the cassette, rather than just half the offspring as expected by Mendelian patterns of inheritance. If a gene drive works efficiently in rapidly reproducing populations such as insects, it would be predicted that an entire population could be manipulated to carry the desired gene on the cassette.<\/p>\n<p>Gene drives have flourished in mosquito studies that have adapted the genetic-engineering tools developed in the fruit fly\u00a0<i>Drosophila melanogaster<\/i>. Gene drives engineered in mosquitoes can be stably transmitted over many generations through a process that uses a form of high-fidelity homologous recombination that is remarkably efficient in the mosquito reproductive tissues (the germ line)<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-00185-y?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR4\">4<\/a><\/sup><sup>,<\/sup><sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-00185-y?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR6\">6<\/a><\/sup>. However, it has been difficult to apply these approaches in mammals, which have evolved independently from insects for more than 700 million years.<\/p>\n<p>But now, Grunwald and colleagues have developed a CRISPR-based gene drive for mice. They engineered animals to express Cas9 and a cassette they called CopyCat, which encoded a guide RNA that targets a sequence in the gene\u00a0<i>Tyr<\/i>\u00a0(Fig. 1). CopyCat was inserted into the\u00a0<i>Tyr<\/i>sequence at a position that ensured that the guide RNA wouldn\u2019t target the copy of\u00a0<i>Tyr\u00a0<\/i>in which the cassette was inserted.<\/p>\n<p><i>Tyr<\/i>\u00a0encodes an enzyme called tyrosinase, which affects mouse coat colour. This enabled the frequency of genetic modification of the gene to be tracked over generations by monitoring coat colour and using DNA-sequence analysis to assess the transmission of the CopyCat cassette. The authors tested the effect of different genetic elements called promoters that affect Cas9 expression patterns. If Cas9 was expressed ubiquitously and continuously, the Cas9-mediated cut site in\u00a0<i>Tyr<\/i>\u00a0had a high level of DNA damage, which arose from a DNA-repair process called non-homologous end joining (NHEJ). When the authors limited Cas9 expression to the male germ line, they also observed high rates of DNA damage caused by NHEJ. However, the gene drive worked successfully when Cas9 was expressed specifically in the female germ line, and, in this context, the Cas9 cuts of the\u00a0<i>Tyr<\/i>\u00a0sequence were repaired by homologous recombination. The transmission rates of the CopyCat element to the next generation in female mice were greater than the 50% transmission that would be expected for standard Mendelian inheritance. The maximum efficiency of this CRISPR editing was a 72% success rate in copying the CopyCat cassette.<\/p>\n<p>The reason for the sex-specific differences in homologous recombination and NHEJ that the authors observed is unknown. But it could be a major impediment for using mammalian gene drives because NHEJ damages the guide-RNA recognition site and therefore blocks the ability to transmit the gene drive. Male and female germ-cell development is substantially different, so further investigation will be needed to learn whether efficient homologous recombination occurs in the male germ line when the timing or pattern of Cas9 expression is altered. Nevertheless, Grunwald and colleagues\u2019 work is an important proof-of-concept that will surely be followed by modifications that might lead to improvements in future mammalian gene drives.<\/p>\n<p>If gene drives become efficient in mammals, one possible way in which they might be used is to tackle pests or disease-causing agents. The eradication of invasive rodents from islands can bring about a dramatic recovery of native ecosystems, but achieving this eradication using current pest-control methods requires Herculean efforts<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-00185-y?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR8\">8<\/a><\/sup>. A mammalian gene drive might provide a powerful alternative. However, eradication is not the desired outcome if a disease-harbouring species is native to a region but has a key role in supporting ecosystem balance<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-00185-y?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR9\">9<\/a><\/sup><sup>,<\/sup><sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-00185-y?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR10\">10<\/a><\/sup>. Native species can harbour organisms, such as the bacterium that causes plague, that are responsible for deadly human diseases. A gene drive engineered to express an antibody to block an infectious agent would protect people from animal-transmitted disease and maintain native species that are essential<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-00185-y?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR9\">9<\/a><\/sup><sup>,<\/sup><sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-019-00185-y?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR10\">10<\/a><\/sup>\u00a0to the ecosystem.<\/p>\n<p>Another possible application of mammalian gene drives is to speed the generation of animal models of disease, because it can be challenging to breed a mouse that has specific combinations of mutations in several genes.<\/p>\n<p>Because gene drives have the potential to alter an entire species, appropriate regulation of this technology is a major concern. Only the most intractable and major health challenges should be considered for possible interventions using gene drives. Any proposed genetic change should be tested to minimize the chances of unintended consequences to the species or the ecosystem. This challenge is particularly daunting for highly mobile species such as the mosquito, which can fly long distances and across national boundaries. Certainly, the use of a gene drive for mosquito-borne diseases such as malaria warrants international efforts that proceed using careful planning and monitoring, and with the engagement of local communities. Nevertheless, it should be remembered that even the best-planned efforts can have unexpected outcomes. A mammalian gene drive might offer a more attractive test case than an insect one for pest eradication or infectious-disease control, because wild mammalian populations can be more easily restricted to a geographic region than can insect populations.<\/p>\n<p>More than 150 years after Mendel\u2019s work illuminated one way in which genetic inheritance can be governed, a powerful tool has emerged to manipulate inheritance in mammals. It seems certain that the promise of continual improvements in gene drives will be matched with even more discussion of how to move forward. The development of this technique to generate a mammalian gene drive is another milestone in this exciting area of research.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<\/div>\n<div class=\"emphasis\">doi: 10.1038\/d41586-019-00185-y<\/div>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>(\uc6d0\ubb38: <a href=\"https:\/\/www.nature.com\/articles\/d41586-019-00185-y?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29\">\uc5ec\uae30<\/a>\ub97c \ud074\ub9ad\ud558\uc138\uc694~)<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>&nbsp; &nbsp; A method for making a version of a gene more likely to be inherited than normal, generating what is called a gene drive,<a href=\"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2580\" class=\"more-link\">(more&#8230;)<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[33,29,30],"tags":[],"class_list":["post-2580","post","type-post","status-publish","format-standard","hentry","category-do-biology","category-lets-do-science","category-recent-science-news"],"aioseo_notices":[],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack-related-posts":[{"id":474,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=474","url_meta":{"origin":2580,"position":0},"title":"Heredity beyond the gene","author":"biochemistry","date":"May 30, 2018","format":false,"excerpt":"\u00a0 \u00a0 (\uc6d0\ubb38) \u00a0 \u00a0 Nick Lane relishes Carl Zimmer\u2019s history of inherited traits in all their messiness, from genes and culture to epigenetics. \u00a0 \u00a0 Human chromosomes and a nucleus in a false-colour image taken by scanning electron microscope.Credit: Power and Syred\/SPL \u00a0 She Has Her Mother\u2019s Laugh: The\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":2529,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2529","url_meta":{"origin":2580,"position":1},"title":"Mitochondrial DNA can be inherited from fathers, not just mothers","author":"biochemistry","date":"January 18, 2019","format":false,"excerpt":"\u00a0 \u00a0 A tenet of elementary biology is that mitochondria \u2014 the cell\u2019s powerhouses \u2014 and their DNA are inherited exclusively from mothers. A provocative study suggests that fathers also occasionally contribute. \u00a0 The DNA of eukaryotic organisms (such as animals, plants and fungi) is stored in two cellular compartments:\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":2234,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2234","url_meta":{"origin":2580,"position":2},"title":"Not Your Mom\u2019s Genes: Mitochondrial DNA Can Come from Dad","author":"biochemistry","date":"December 3, 2018","format":false,"excerpt":"\u00a0 \u00a0 A new study provides compelling evidence that children can inherit mitochondrial DNA from both their parents. \u00a0 A new study shows that, in contrast to a longstanding rule in human biology, mitochondrial DNA can be inherited from fathers as well as mothers. Photo Credit: seal1837, Pixabay \u00a0 The\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/163.180.4.222\/lab\/wp-content\/uploads\/2018\/12\/baby-2436661_1920.width-2500-300x169.jpg?resize=350%2C200","width":350,"height":200},"classes":[]},{"id":1084,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=1084","url_meta":{"origin":2580,"position":3},"title":"Controversial CRISPR \u2018gene drives\u2019 tested in mammals for the first time","author":"biochemistry","date":"July 10, 2018","format":false,"excerpt":"\u00a0 \u00a0 https:\/\/www.nature.com\/articles\/d41586-018-05665-1?utm_source=feedburner&utm_medium=feed&utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29 \u00a0 \u00a0 \u00a0 Experiments in mice suggest that the technology has a long way to go before being used for pest control in the wild. \u00a0 \u00a0 \u00a0 Mice are the first mammals in which gene-drive technology has been tested.Credit: Stuart Wilson\/Science Photo Library \u00a0 \u00a0 A\u2026","rel":"","context":"In \"Uncategorized\"","block_context":{"text":"Uncategorized","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?tag=uncategorized"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":3625,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=3625","url_meta":{"origin":2580,"position":4},"title":"Principles of and strategies for germline gene therapy","author":"biochemistry","date":"June 4, 2019","format":false,"excerpt":"\u00a0 \u00a0 Abstract Monogenic disorders occur at a high frequency in human populations and are commonly inherited through the germline. Unfortunately, once the mutation has been transmitted to a child, only limited treatment options are available in most cases. However, means of correcting disease-causing nuclear and mitochondrial DNA mutations in\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":1313,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=1313","url_meta":{"origin":2580,"position":5},"title":"Did CRISPR really fix a genetic mutation in these human embryos?","author":"biochemistry","date":"August 9, 2018","format":false,"excerpt":"\u00a0 \u00a0 (\uc6d0\ubb38) \u00a0 \u00a0 Researchers provide more evidence for their landmark claim that gene editing rid embryos of a disease mutation \u2014 but scientists are still arguing over the results. \u00a0 \u00a0 Eight-cell embryos injected with the gene editor CRISPR\u2013Cas9.Credit: H. Ma et al.\/Nature \u00a0 \u00a0 \u00a0 Biologists who\u2026","rel":"","context":"In &quot;Essays on Science&quot;","block_context":{"text":"Essays on Science","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=32"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]}],"jetpack_sharing_enabled":false,"jetpack_shortlink":"https:\/\/wp.me\/p9Xo1j-FC","_links":{"self":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts\/2580","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2580"}],"version-history":[{"count":1,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts\/2580\/revisions"}],"predecessor-version":[{"id":2581,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts\/2580\/revisions\/2581"}],"wp:attachment":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2580"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2580"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2580"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}