{"id":2012,"date":"2018-10-05T11:44:41","date_gmt":"2018-10-05T02:44:41","guid":{"rendered":"http:\/\/163.180.4.222\/lab\/?p=2012"},"modified":"2018-10-05T11:44:41","modified_gmt":"2018-10-05T02:44:41","slug":"the-eukaryotic-ancestor-shapes-up","status":"publish","type":"post","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2012","title":{"rendered":"The eukaryotic ancestor shapes up"},"content":{"rendered":"<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>\uc9c4\ud575\uc138\ud3ec\uc758 \uc870\uc0c1\uc5d0 \uad00\ud55c \ub0b4\uc6a9\uc785\ub2c8\ub2e4.<\/p>\n<p>(\uc6d0\ubb38: <a href=\"https:\/\/www.nature.com\/articles\/d41586-018-06868-2?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29\"><u>\uc5ec\uae30<\/u><\/a>\ub97c \ud074\ub9ad\ud558\uc138\uc694~)<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h5>Asgard archaea are the closest known relatives of nucleus-bearing organisms called eukaryotes. A study indicates that these archaea have a dynamic network of actin protein \u2014 a trait thought of as eukaryote-specific.<\/h5>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<div class=\"article__body serif cleared\">\n<p>Eukaryotic cells, which carry their DNA in a nucleus, are thought to have evolved from a merger between two other organisms \u2014 an archaeal host cell<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-018-06868-2?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR1\">1<\/a><\/sup><sup>\u2013<\/sup><sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-018-06868-2?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR3\">3<\/a><\/sup>\u00a0and a bacterium from which eukaryotic organelles called mitochondria emerged<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-018-06868-2?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR4\">4<\/a><\/sup>. Some insights into the biological properties of the host have come from the closest known archaeal relatives of eukaryotes, the Asgard superphylum<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-018-06868-2?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR5\">5<\/a><\/sup><sup>,<\/sup><sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-018-06868-2?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR6\">6<\/a><\/sup>. The genomes of organisms belonging to this archaeal group encode a suite of proteins typically involved in functions or processes thought to be eukaryote-specific. The functions of these \u2018eukaryotic genes\u2019 in Asgard archaea have been elusive, but in\u00a0<a href=\"https:\/\/www.nature.com\/articles\/s41586-018-0548-6\" data-track=\"click\" data-label=\"https:\/\/www.nature.com\/articles\/s41586-018-0548-6\" data-track-category=\"body text link\">a paper in\u00a0<i>Nature<\/i><\/a>, Ak\u0131l and Robinson<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-018-06868-2?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR7\">7<\/a><\/sup>\u00a0provide evidence that some of them encode proteins that are structurally and functionally similar to their eukaryotic counterparts.<\/p>\n<p>&nbsp;<\/p>\n<aside class=\"recommended pull pull--left sans-serif\" data-label=\"Related\"><a href=\"https:\/\/www.nature.com\/articles\/s41586-018-0548-6\" data-track=\"click\" data-track-label=\"recommended article\"><img decoding=\"async\" class=\"recommended__image\" src=\"https:\/\/media.nature.com\/w400\/magazine-assets\/d41586-018-06868-2\/d41586-018-06868-2_16156094.jpg\" \/><\/a><\/p>\n<p class=\"recommended__title serif\">Read the paper: Genomes of Asgard archaea encode profilins that regulate actin<\/p>\n<\/aside>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>Apart from their nucleus and energy-producing mitochondria, eukaryotic cells are characterized by a complex internal system of membrane-bound compartments (the endomembrane system), and by a dynamic network of proteins such as actin, called the cytoskeleton. The latter gives the cells their shape and structure, but is also involved in a variety of cellular processes specific to eukaryotes<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-018-06868-2?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR8\">8<\/a><\/sup>. These features are thought to have been present in the last common ancestor of all eukaryotes, which lived about 1.8 billion years ago<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-018-06868-2?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR9\">9<\/a><\/sup>, but no life forms have been found that represent an intermediate between eukaryotes and their bacterial and archaeal ancestors. The seemingly sudden emergence of cellular complexity in the eukaryotic lineage is a conundrum for evolutionary biologists.<\/p>\n<p>Several of the proteins produced by Asgard archaea are evolutionarily related to proteins that in eukaryotes modulate complex cellular processes<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-018-06868-2?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR5\">5<\/a><\/sup><sup>,<\/sup><sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-018-06868-2?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR6\">6<\/a><\/sup>. The identification of these proteins raised the question of whether Asgard archaea have some primitive versions of certain eukaryotic properties. If they do, it would suggest that the last archaeal ancestor of eukaryotes already displayed a certain \u2014 albeit probably limited \u2014 degree of cellular complexity reminiscent of eukaryotes.<\/p>\n<p>Experiments to support such ideas are complicated by the fact that evidence for the existence of the four known Asgard lineages (Lokiarchaeota, Odinarchaeota, Thorarchaeota and Heimdallarchaeota)<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-018-06868-2?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR5\">5<\/a><\/sup><sup>,<\/sup><sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-018-06868-2?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR6\">6<\/a><\/sup>\u00a0is based solely on metagenomics analyses. The cells have yet to be observed under a microscope, and have not been cultured\u00a0<i>in vitro<\/i>. Nevertheless, Ak\u0131l and Robinson were determined to gain insight into the properties of Asgard proteins related to the eukaryotic proteins actin and profilin. In eukaryotes, profilin regulates the polymerization of actin into filaments of the cytoskeleton. These filaments have pivotal roles in processes that include vesicle and organelle movement, cell-shape formation and phagocytosis<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-018-06868-2?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR8\">8<\/a><\/sup>, in which cells ingest foreign particles or other cells.<\/p>\n<p>To produce Asgard profilins, Ak\u0131l and Robinson expressed these proteins in the bacterium\u00a0<i>Escherichia coli<\/i>\u00a0using a circular DNA molecule called a plasmid that harboured the profilin-encoding genes. They then purified the proteins and studied their structures using X-ray crystallography. Asgard profilins share limited amino-acid sequence identity with their eukaryotic counterparts. Nonetheless, the authors found that the structure of lokiarchaeal profilin is topologically similar to that of human profilin, although some structural divergences could be observed. This confirms that Asgard and eukaryotic profilins are indeed evolutionarily related, albeit distantly.<\/p>\n<p>Next, the researchers set out to investigate whether Asgard profilins could interact with Asgard actins. Unfortunately, despite considerable efforts, they were unable to produce functional Asgard actin. As an alternative, they therefore carried out\u00a0<i>in vitro<\/i>\u00a0and co-crystallization experiments to test whether Asgard profilins could interact with eukaryotic actins. Remarkably, despite being separated by 2 billion to 3 billion years of evolution<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-018-06868-2?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR9\">9<\/a><\/sup>, several of the Asgard profilins bound to mammalian actin and regulated its polymerization kinetics. Asgard and mammalian profilins seem to have similar effects on mammalian actin, although the Asgard proteins act less efficiently. These results suggest that Asgard archaea harbour a profilin-regulated actin cytoskeleton \u2014 a cellular feature generally regarded as a defining characteristic of eukaryotic cells (Fig. 1).<\/p>\n<p>&nbsp;<\/p>\n<figure class=\"figure\">\n<div class=\"embed intensity--high\">\n<div class=\"embed intensity--high\"><img decoding=\"async\" class=\"figure__image\" src=\"https:\/\/media.nature.com\/w800\/magazine-assets\/d41586-018-06868-2\/d41586-018-06868-2_16172590.jpg\" alt=\"\" data-src=\"\/\/media.nature.com\/w800\/magazine-assets\/d41586-018-06868-2\/d41586-018-06868-2_16172590.jpg\" \/><\/div>\n<\/div><figcaption>\n<p class=\"figure__caption sans-serif\"><span class=\"mr10\"><b>Figure 1 | Cellular complexity along the tree of life.<\/b>\u00a0The Eukarya (organisms whose cells harbour DNA in a nucleus) are thought to have arisen from a merger between their last archaeal ancestor and a bacterium. In addition to a nucleus, eukaryotes have several characteristics that are thought to separate them from archaea, including: a complex internal system of membranes called endomembranes; a structural feature called the actin cytoskeleton, the dynamics of which are regulated by the protein profilin; and energy-producing organelles called mitochondria, which arose from the bacterial partner. But Ak\u0131l and Robinson<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-018-06868-2?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR7\">7<\/a><\/sup>provide evidence that members of the Asgard superphylum \u2014 an extant group of archaea thought to be related to eukaryotes \u2014 harbour a primitive profilin-regulated actin cytoskeleton. If the last archaeal ancestor of eukaryotes had this feature, it might have enabled the cell to wrap around its presumed bacterial partner. In addition, it is possible that Asgard archaea and the last archaeal ancestor of eukaryotes carry primitive endomembrane systems. (Cells and cellular features are not drawn to scale.)<\/span><\/p>\n<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>The inference of a primitive dynamic actin cytoskeleton in Asgard archaea sheds light on the biological properties of the ancestor of eukaryotes. In eukaryotic cells, the energy required to dynamically regulate actin is mainly provided by mitochondria<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-018-06868-2?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR10\">10<\/a><\/sup>. Although the energetic and metabolic properties of Asgard archaea are currently unknown, they certainly lack the firepower that mitochondria provide. A profilin-regulated actin cytoskeleton in the archaeal ancestor of eukaryotes is therefore unlikely to sustain energy-consuming processes such as phagocytosis.<\/p>\n<p>But was the energy provided by mitochondria necessarily the ultimate driving force for the emergence of complex cellular features in eukaryotes? Archaea such as\u00a0<i>Ignicoccus hospitalis<\/i>, along with several types of bacterium, have independently evolved endomembrane systems<sup><a href=\"https:\/\/www.nature.com\/articles\/d41586-018-06868-2?utm_source=feedburner&amp;utm_medium=feed&amp;utm_campaign=Feed%3A+nature%2Frss%2Fcurrent+%28Nature+-+Issue%29#ref-CR11\">11<\/a><\/sup>. Because these lineages lack mitochondria, energetic constraints can be ruled out as a limiting factor in the emergence of such a system. It is therefore feasible that Asgard archaeal cells produce sufficient energy to harbour both a primitive endomembrane system and undergo actin-driven membrane and cell-shape deformation. Perhaps the latter ability could have facilitated the symbiotic interaction between the Asgard-related host cell and the bacterial ancestor of mitochondria, for example by optimizing the membrane surface area for metabolic exchange between the two cells. Once mitochondria became an intrinsic part of eukaryotic cells, their capacity for energy production could have conferred selective advantages on their host. However, the exact contribution of these organelles to the emergence of the complex features of eukaryotic cells remains unresolved.<\/p>\n<p>Future efforts to elucidate the biological and physiological properties of Asgard archaea will be essential to increase our understanding of the emergence of eukaryotes. Although biochemical and structural studies of individual Asgard proteins, such as those by Ak\u0131l and Robinson, are likely to provide piecemeal insights, it is the ability to grow Asgard archaeal lineages\u00a0<i>in vitro<\/i>\u00a0that will ultimately unravel their obscure biology.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<\/div>\n<div class=\"emphasis\">doi: 10.1038\/d41586-018-06868-2<\/div>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>&nbsp; &nbsp; \uc9c4\ud575\uc138\ud3ec\uc758 \uc870\uc0c1\uc5d0 \uad00\ud55c \ub0b4\uc6a9\uc785\ub2c8\ub2e4. (\uc6d0\ubb38: \uc5ec\uae30\ub97c \ud074\ub9ad\ud558\uc138\uc694~) &nbsp; &nbsp; Asgard archaea are the closest known relatives of nucleus-bearing organisms called eukaryotes. A study<a href=\"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2012\" class=\"more-link\">(more&#8230;)<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[32,33,29],"tags":[],"class_list":["post-2012","post","type-post","status-publish","format-standard","hentry","category-essays-on-science","category-do-biology","category-lets-do-science"],"aioseo_notices":[],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack-related-posts":[{"id":3532,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=3532","url_meta":{"origin":2012,"position":0},"title":"The trickster microbes that are shaking up the tree of life","author":"biochemistry","date":"May 16, 2019","format":false,"excerpt":"\u00a0 \u00a0 Mysterious groups of archaea \u2014 named after Loki and other Norse myths \u2014 are stirring debate about the origin of complex creatures, including humans. \u00a0 Illustration by Fabio Buonocore \u00a0 \u00a0 Every mythology needs a good trickster, and there are few better than the Norse god Loki. He\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":2647,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2647","url_meta":{"origin":2012,"position":1},"title":"Algae suggest eukaryotes get many gifts of bacteria DNA","author":"biochemistry","date":"February 8, 2019","format":false,"excerpt":"\u00a0 \u00a0 Algae found in thermal springs and other extreme environments have heated up a long-standing debate: Do eukaryotes\u2014organisms with a cell nucleus\u2014sometimes get an evolutionary boost in the form of genes transferred from bacteria? The genomes of some red algae, single-celled eukaryotes, suggest the answer is yes. About 1%\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":2712,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2712","url_meta":{"origin":2012,"position":2},"title":"DNA replication from two different worlds","author":"biochemistry","date":"February 22, 2019","format":false,"excerpt":"\u00a0 \u00a0 Replication of the DNA genome is performed by a replisome complex composed of numerous proteins. Cells have duplex DNA genomes, and their replisomes duplicate both strands simultaneously. A functional replisome requires, at a minimum, a helicase to unwind the DNA duplex, two DNA polymerases (Pols) to replicate the\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":3895,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=3895","url_meta":{"origin":2012,"position":3},"title":"CRISPR patent fight revived","author":"biochemistry","date":"July 16, 2019","format":false,"excerpt":"\u00a0 \u00a0 A surprise ruling last week reignited the high-profile patent fight over who invented a key application of the genome editor CRISPR. The 3-year-old battle pits parties represented by the University of California (UC) against the Broad Institute in Cambridge, Massachusetts. It revolves around the use of CRISPR, originally\u2026","rel":"","context":"In &quot;Essays on Science&quot;","block_context":{"text":"Essays on Science","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=32"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":2985,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2985","url_meta":{"origin":2012,"position":4},"title":"How to make an organelle in eukaryotes","author":"biochemistry","date":"March 29, 2019","format":false,"excerpt":"\u00a0 \u00a0 A key step in the evolution of complex organisms like eukaryotes was the organization of specific tasks into organelles. Reinkemeier\u00a0et al.\u00a0designed an artificial, membraneless organelle into mammalian cells to perform orthogonal translation. In response to a specific codon in a selected messenger RNA, ribosomes confined to this organelle\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":2545,"url":"https:\/\/biochemistry.khu.ac.kr\/lab\/?p=2545","url_meta":{"origin":2012,"position":5},"title":"G\ufeffaps in our genes are more important than we thought","author":"biochemistry","date":"January 19, 2019","format":false,"excerpt":"\u00a0 \u00a0 Introns, the bits of non-coding DNA scattered through our genes, may play an important role in cell survival \u00a0 \u00a0 \u00a0 Introns are short stretches of non-coding DNA interspersed with the coding DNA in the genes of eukaryotic organisms. They are widespread and common but their evolutionary benefit\u2026","rel":"","context":"In &quot;Let's Do Biology!&quot;","block_context":{"text":"Let's Do Biology!","link":"https:\/\/biochemistry.khu.ac.kr\/lab\/?cat=33"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]}],"jetpack_sharing_enabled":false,"jetpack_shortlink":"https:\/\/wp.me\/p9Xo1j-ws","_links":{"self":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts\/2012","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2012"}],"version-history":[{"count":1,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts\/2012\/revisions"}],"predecessor-version":[{"id":2013,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=\/wp\/v2\/posts\/2012\/revisions\/2013"}],"wp:attachment":[{"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2012"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2012"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biochemistry.khu.ac.kr\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2012"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}