![\"Lab](\"https:\/\/media.nature.com\/w800\/magazine-assets\/d41586-018-05665-1\/d41586-018-05665-1_15913324.jpg\")
<\/div>\n<\/div>Mice are the first mammals in which gene-drive technology has been tested.<\/span>Credit: Stuart Wilson\/Science Photo Library<\/p>\n<\/figcaption><\/figure>\n <\/p>\n <\/p>\n A controversial technology capable of altering the genomes of entire species has been applied to mammals for the first time. In a preprint posted1<\/a><\/sup>\u00a0to bioRxiv on 4 July, researchers describe developing \u2018gene drives\u2019 \u2014 which could be used to eradicate problematic animal populations \u2014 in lab mice using the CRISPR gene-editing technique.<\/p>\n Gene drives ensure that chosen mutations are passed onto nearly all an animal\u2019s offspring. They have already\u00a0been created in mosquitoes in the lab<\/a>, as a potential malaria-control strategy. Researchers have raised the possibility that the technology could help to kill off invasive rats, mice and other rodent pests. But the latest study dashes hopes of that happening anytime soon, say scientists. The technique worked inconsistently in lab mice, and myriad technological hurdles remain before researchers could even consider releasing the tool in the wild.<\/p>\n \u201cThere\u2019s an indication it could work, but it\u2019s also sobering,\u201d says Paul Thomas, a development geneticist at the University of Adelaide in Australia, who was not involved in the research. \u201cIt\u2019s a lot longer to go where you could consider gene drives for a useful tool for population control of rodents.\u201d His lab is doing similar work, as part of an international consortium to use gene drives to combat invasive rodents.<\/p>\n Gene drives work by ensuring that a higher proportion of an organism\u2019s offspring inherit a certain, \u2018selfish\u2019 gene than would happen by chance, allowing a mutation or foreign gene to spread quickly through a population. They occur naturally in some animals, including mice, where they can cause death or infertility. But\u00a0the revolutionary CRISPR\u2013Cas9 gene-editing tool<\/a>\u00a0has led to the development of synthetic gene drives that are designed to eliminate problem species, such as malaria-transmitting mosquitoes, from the wild by, for instance, ensuring that offspring are infertile. The technology has attracted controversy \u2014\u00a0and even a failed attempt to ban its global use<\/a>\u00a0\u2014 because, if released in the wild, organisms carrying gene drives might be hard to contain.<\/p>\n A team led by Kim Cooper, a developmental geneticist at the University of California, San Diego, did not attempt to develop a gene drive to make lab mice (Mus musculus<\/i>) infertile. Rather, their goal was to create a test-bed for the technology, which they say could also be useful in basic research: they biased the inheritance of a mutation that gives mice all-white coats, instead of infertility.<\/p>\n CRISPR-based gene drives use the gene-editing tool to copy a mutation on one chromosome to the second of a pair, usually during an animal\u2019s early development. When Cooper\u2019s team attempted this in mice embryos, the mutation was not always copied correctly, and it worked only in female embryos.<\/p>\n Based on those results, her team estimated that this could lead to a mutation being transmitted to about 73% of a female mouse\u2019s offspring, on average, instead of the usual 50% for most genes operating under the usual rules of inheritance. Cooper declined to comment on her team\u2019s work, because it has not yet been published in a peer-reviewed journal.<\/p>\n Tony Nolan, a molecular biologist at Imperial College London who is part of a team developing gene drives in malaria-carrying mosquitoes, is excited to see that gene drives can, at least, work in rodents. Even if the technology doesn’t become an eradication tool, it could help to produce transgenic lab animals that model diseases caused by multiple mutations more efficiently than existing technologies, he says.<\/p>\n Other researchers agree that the study is important, but say it also shows just how long the technology has to go in rodents. \u201cCould you imagine this gene drive in the wild? That\u2019s not going to happen,\u201d says Ga\u00e9tan Burgio, a geneticist who works on CRISPR at Australia National University in Canberra. The relatively low efficiency of the technique means it would take many generations for the gene drive to spread through an entire rodent population, leaving ample time for\u00a0species to evolve resistance to the gene drive<\/a>.<\/p>\n Thomas describes the results as a \u201creality check\u201d for efforts to develop gene drives in rodents. \u201cIt gives an indication to how much further there is to go,\u201d he says. Future work should seek to improve efficiency, as well as understand why the technique doesn\u2019t work in male mice, Thomas adds.<\/p>\n He is a member of a consortium called Genetic Biocontrol of Invasive Rodents, or GBIRd, that hopes to deploy gene drives against invasive rodents.<\/p>\n CRISPR gene drives aren\u2019t the consortium\u2019s only strategy to deal with invasive rodents. GBIRd member and geneticist David Threadgill, and his team at Texas A&M University in College Station, are working with a gene drive that occurs naturally in mice, called the t-haploype. The researchers plan to modify this selfish gene to create daughterless mice: females carrying two copies will give birth only to males, potentially resulting in an eventual population crash.<\/p>\n Should gene-drive technology prove effective at controlling rodents, islands are an ideal testbed, says Heath Packard, director of Island Conservation, a GBIRd partner that focuses on eradicating invasive pests. Rodent\u00a0pesticides that have eliminated problem mice and rats on small islands<\/a>\u00a0are too risky to use on larger islands, with complex ecosystems and large human populations, Packard says. Gene drives, which could be contained on islands, are still a technology worth investigating. \u201cWe\u2019re hopeful that this might be a tool that could serve the island restoration community,\u201d he says, \u201cbut we don\u2019t know if it\u2019s going to work.\u201d<\/p>\n <\/p>\n <\/p>\n<\/div>\n